摘要
2016年1月-2021年12月,南京市急性胃肠炎暴发主要由GII.2[P16]引起,但2021年11月底,由GII.4Sydney2012[P16]引起的暴发显著上升。为了解GII.4 Sydney2012[P16]分子生物学特性,对监测期间204起急性胃肠炎暴发中收集到的3129份样本进行荧光定量PCR检测,检出1006份诺如病毒阳性样本,对部分阳性样进行一步法RT-PCR扩增、测序,共检出10种GII型诺如病毒,随机选取19株GII.4 Sydney2012[P16],结合7株GII.4Sydney2012[P31]、31株GII.2[P16]南京株及2015-2021年国内外参考株进行序列比对和进化分析。19株GII.4Sydney2012[P16]同源性达99.7%~100.0%,与参考株同源性达96.6%~99.3%。进化树分析显示,GII.P16虽与不同GII型诺如病毒组合,但进化距离上并未发生较大变化。与不同聚合酶区结合的GII.4 Sydney 2012在同一分支上,GII.4 Sydney 2012[P16]在进化距离上与GII.4 Sydney 2012[P4 NewOrleans2009]更为接近。P2区的A、B、D和NERK位点均发生改变,所有GII.4的373氨基酸残基处均检测到显著的阳性选择。GII.P16虽未发生较大改变,但通过与GII.4 Sydney2012重组,呈现加速在人群中流行的分子特征。而GII.4 Sydney2012通过改变抗原表位位点影响了抗原性和配体结合特性,显著促进了病毒的传播。因此GII.4 Sydney2012[P16]是否会取代GII.2[P16]成为南京地区流行株,抑或被其他GII.4所替代,今后需要对更为广泛的潜在感染人群进行监测,及早发现早期新型诺如病毒。
From January 2016 to November 2021,the outbreak of acute gastroenteritis in Nanjing,China,was mainly caused by GII norovirus,of which GII.2[P16]was the main epidemic strain.However,from the end of November 2021,the outbreak caused by GII.4 Sydney[P16]increased significantly.In order to understand the molecular biological characteristics of GII.4 Sydney[P16],a total of 3,129 samples collected from 204 acute gastroenteritis outbreaks during the surveillance period were detected by fluorescence quantitative PCR,and1,006 norovirus positive samples were detected.By one-step reverse-transcription-PCR(RT-PCR)amplification and sequencing,a total of 10 GII norovirus types were detected.19 GII.4 Sydney[P16]were randomly selected and combined with 7 GII.4 Sydney[P31],31 GII.2[P16]Nanjing strains and reference strains at home and abroad from 2015 to 2021 for sequence comparison and evolution analysis.The homology of19 GI.4 Sydney[P16]was 99.7%-100.0%,and 96.6%-99.3%compared with reference strains.Phylogenetic tree analysis showed that although GII.P16 recombined with many different types of GII norovirus,there was no significant change in evolutionary distance.GII.4 Sydney2012[P16]is closer to GII.4 Sydney2012[P4NewOrleans2009]in evolutionary distance.Sites A,B,D and NERK in P2 region were altered,and significant positive selection was detected at site 373 in GII.4.In this study,it was found that GII.P16 as a polymerase domain did not change significantly,but by recombination with GII.4 Sydney,GII.4 Sydney2012[P16]showed the molecular characteristics of accelerating epidemic in the population.However,GII.4 Sydney influenced antigenicity and ligand binding properties by changing antigenic epitopes,which significantly promoted viral transmission.Therefore,whether GII.4 Sydney[P16]will replace GII.2[P16]as an epidemic strain in Nanjing or be replaced by other GII.4 strains,it is necessary to monitor a wider range of potential infected population in the future for early detection new norovirus.
作者
王璇
雍玮
刘品
斯佳丽
龚红瑾
丁洁
WANG Xuan;YONG Wei;LIU Pin;SI Jiali;GONG Hongjin;DING Jie(Nanjing Municipal Center for Disease Control and Prevention,Nanjing 210003,China)
出处
《病毒学报》
CAS
CSCD
北大核心
2022年第6期1305-1314,共10页
Chinese Journal of Virology
基金
江苏省卫生健康委医学科研立项项目(项目号:H2019019),题目:诺如病毒深度测序与分子溯源技术研究及应用。
