摘要
炭疽病是菜用大豆生产上发生的常见真菌性病害,破坏力强,发病时在豆荚表面形成黑色斑点,严重影响鲜荚外观商品性和品质,已成为我国菜用大豆生产上面临的主要问题之一。为明确浙江省菜用大豆炭疽病病原菌的种类,本文对采集的菜用大豆炭疽病样本进行分离纯化培养,基于柯赫氏法则分离到引起菜用大豆炭疽病的病原菌,并通过病原菌的菌落形态特征结合其rDNA-ITS区域的序列分析对病原菌进行种类鉴定。结果表明,造成浙江省菜用大豆炭疽病的主要病原菌为平头炭疽菌(Colletotrichum truncatum)和胶孢炭疽菌(Colletotrichum gloeosporioides)。为筛选可以用于防控菜用大豆炭疽病的杀菌剂,测定了分离得到的病原菌平头炭疽菌Cts18和胶孢炭疽菌Cts22对生产中常用杀菌剂多菌灵和戊唑醇的敏感性,发现多菌灵对Cts18、Cts22的抑制中浓度(EC_(50))分别为2.13μg·mL^(-1)和96.12μg·mL^(-1),戊唑醇对Cts18、Cts22的EC_(50)分别为0.27μg·mL^(-1)和0.63μg·mL^(-1)。结果表明,平头炭疽菌Cts18相比胶孢炭疽菌Cts22对多菌灵和戊唑醇更加敏感,其中戊唑醇可作为生产中防控菜用大豆炭疽病的杀菌剂之一。
Anthracnose disease is a common fungal disease of vegetable soybean,which forms black spots on the pod surface and affects the appearance and quality of fresh pod seriously.It has become one of economically important fungal diseases on vegetable soybean yield in China.To identify the pathogens causing vegetable soybean anthracnose in Zhejiang Province,we collected anthracnose disease samples and isolated the pathogens according to Koch's postulate.The pathogens were further identified based on morphological characteristics and rDNA-ITS sequence analysis in this study.Our results showed that two Colletotrichum species,Colletotrichum truncatum and Colletotrichum gloeosporioides,were the major pathogens causing vegetable soybean anthracnose in this region.To further guide the vegetable soybean anthracnose control using fungicides,we measured the sensitivity of representative strain C.truncatum Cts18 and C.Gloeosporioides Cts22 to commonly used fungicide carbendazim and tebuconazole.Results indicated that the concentration for 50%of maximal effect(EC_(50))of carbendazim against C.truncatum Cts18 and C.gloeosporioides Cts22 were 2.13μg·mL^(-1) and 96.12μg·mL^(-1),respectively.The EC_(50) of tebuconazole against Cts18 and Cts22 were 0.27μg·mL^(-1) and 0.63μg·mL^(-1),respectively.These results suggested that Cts18 was more sensitive than Cts22 towards the tested fungicides,and tebuconazole is a potential fungicide for managing vegetable soybean anthracnose in fields in this region.
作者
刘娜
范翘楚
周佳
宋雅静
张古文
冯志娟
卜远鹏
王斌
龚亚明
LIU Na;FAN Qiaochu;ZHOU Jia;SONG Yajing;ZHANG Guwen;FENG Zhijuan;BO Yuanpeng;WANG Bin;GONG Yaming(State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products,Institute of Vegetables,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)
出处
《浙江农业学报》
CSCD
北大核心
2022年第12期2682-2688,共7页
Acta Agriculturae Zhejiangensis
基金
农产品质量安全危害因子与风险防控国家重点实验室自主设计课题(2010DS700124-ZZ2005)
浙江省重点研发课题(2021C02052)
浙江省“十四五”蔬菜育种专项(2021C02065-6)
浙江省基础公益研究计划(LGN20C150006)
国家自然科学基金(31872114)。
关键词
菜用大豆
炭疽病
病原菌鉴定
平头炭疽菌
胶孢炭疽菌
杀菌剂敏感性
vegetable soybean
anthracnose
pathogen identification
Colletotrichum truncatum
Colletotrichum gloeosporioides
fungicide sensitivity
