摘要
该文探索机体可能暴露剂量的重组人金属硫蛋白Ⅲ(rh-MT-Ⅲ)对人永生化角质形成细胞(human keratinocytes,HaCaT)、小鼠成纤维细胞(L929)和秀丽隐杆线虫(Caenorhabdities elegans,C.elegans)的毒性效应,并探究其安全剂量范围,为安全应用提供实验依据。以0、12.5、25、50、100和200μg/mL rh-MT-Ⅲ对HaCaT细胞和L929细胞暴露24 h,检测其对细胞存活率、形态学变化、细胞膜损伤程度和细胞凋亡水平的影响;以0、5、50、500μg/mL rh-MT-Ⅲ对L1期线虫暴露72 h,考察其对线虫存活率、运动行为(头部摆动和身体弯曲频率)、生长发育(体长和体宽)、摄食和排便行为的影响。结果显示,与对照组相比,两种细胞的存活率、细胞形态、乳酸脱氢酶释放率及凋亡水平以及线虫各项检测指标,在研究剂量下均无统计学差异。这表明rh-MT-Ⅲ在0~200μg/mL剂量时对HaCaT细胞和L929细胞无明显毒性效应;0~500μg/mL对线虫无明显毒性效应。
This study aimed to investigate the toxic effects of different concentrations of rh-MT-Ⅲ(recombinant human metallothionein Ⅲ)on HaCaT(human keratinocytes)and L929 cells as well as on C.elegans(Caenorhabdities elegans).The functional data of safety and toxicity,including cell viabilities,cell morphology,cell membrane damages,apoptosis levels of HaCaT and L929 cells,and nematode survival,head swinging and body bending frequency,body length and width,pharyngeal pumping frequency and defecation interval of C.elegans were observed,respectively.For the cell experiments,cells were exposed to rh-MT-Ⅲ solution at concentrations ranging from 0μg/mL,12.5μg/mL,25μg/mL,50μg/mL,100μg/mL,200μg/mL for 24 h.For the nematode experiments,0μg/mL,5μg/mL,50μg/mL,500μg/mL rh-MT-Ⅲ were used to treat L1 phase nematodes for 72 h.The results showed that there was no significant difference of the functional data between the rh-MT-Ⅲ treatment and the control groups.These results indicated that rh-MT-Ⅲat doses of 0~200μg/mL and 0~500μg/mL had no obvious toxicity to both two cells and C.elegans,respectively.
作者
孙左义
原海亮
秦建新
李强
薛玉英
SUN Zuoyi;YUAN Hailiang;QIN Jianxin;LI Qiang;XUE Yuying(Key Laboratory of Environmental Medicine and Engineering,Ministry of Education,School of Public Health,Southeast University,Nanjing 210009,China;Suzhou Hvha Medical Technology Development Co.,Ltd.,Changshu 215500,China;Changshu Municipal Market Supervision Administration,Changshu 215500,China)
出处
《中国细胞生物学学报》
CAS
CSCD
2022年第7期1339-1348,共10页
Chinese Journal of Cell Biology
