摘要
目的从微生物角度探究重度低龄儿童龋(severe early childhood caries,S⁃ECC)患儿一次完成龋病治疗前、治疗后3个月内菌斑微生物群落构成和多样性的变化及治疗对于远期无龋状态维持的作用。方法S⁃ECC患儿在全身麻醉下一次完成龋病治疗,采集治疗前(C)、术后7 d(C⁃7D)、1个月(C⁃1M)、3个月(C⁃3M)无龋牙面集合菌斑,并纳入无龋儿童(CF)为对照组,分析治疗前后菌斑微生物群落短期内的动态改建过程。结果S⁃ECC组和CF组菌斑群落组成高度相似;组间α多样性指数差异无统计学意义(P>0.05);从相对丰度值分析,纤毛菌属、聚集杆菌属等在治疗后较术前下降(P<0.05),C⁃7D组血链球菌较C组上升并在3个月内逐渐下降;治疗前韦荣菌属、放线菌属、拟普雷沃菌属、二氧化碳嗜纤维菌属、变异链球菌在C组和CF组间存在显著差异(P<0.05),其中变异链球菌经治疗后的C⁃7D、C⁃1M组与CF组未存在显著差异,而C⁃3M组较CF组出现上升(P<0.01)。结论S⁃ECC患儿在接受治疗后菌群结构的迅速改变,并在治疗后1~3个月逐渐开始建立新的无龋状态下的平衡。核心微生物群的存在使得菌斑群落保持稳定,韦荣菌属、拟普雷沃菌属、变异链球菌等具备作为龋病菌斑微生物标记物潜能。
Objective To explore from the perspective of microorganisms the changes in plaque microbial commu⁃nity of children with severe early childhood caries(S-ECC)before and 3 months after dental treatment.Meanwhile to show the effect of treatment on the maintenance of long-term caries-free state.Methods S⁃ECC children completed dental treatment under general anesthesia.We collected plaque from caries⁃free dental surfaces before treatment(car⁃ies,C)and at the postoperative follow⁃up review time points of 7 days(C⁃7D),1 month(C⁃1 M),and 3 months(C⁃3 M).We included caries⁃free children(caries free,CF)as the control group to analyze the dynamic modification process of the plaque microbial community in the short⁃term pre⁃and postdental treatment.Results Species clustering analysis showed that the compositions of the microbial communities of the S⁃ECC and CF groups were highly similar.Theαdi⁃versity index was not statistically significant(P>0.05).From the analysis of the relative abundance,Leptotrichia spp.and Aggregatibacter spp.decreased after treatment compared with before treatment(P<0.05).Streptococcus sanguinis in the C⁃7D group increased compared with that in the C group and gradually decreased within 3 months.Veillonella spp.,Actinomyces spp.,Allprevotella spp.,Capnocytophaga spp.,and Streptococcus mutans differed between the C and CF groups(P<0.05),Streptococcus mutans did not differ significantly between the C⁃7D and C⁃1 M groups and the CF group after treatment,while C⁃3 M showed an increase compared with the CF group(P<0.01).Conclusion The rap⁃id change in the structure of the flora of children with S⁃ECC after treatment.The plaque microbial community struc⁃ture in a caries⁃free state gradually starts to be established 1⁃3 months after treatment.There is a"core microbiota"in the oral plaque community that jointly maintains microecological stability.Veillonella spp.,Allprevotella spp.and Strepto⁃coccus mutans have potential as possible microbial markers.
作者
周庆楠
赵焕英
蔡爽
杨宁燕
周焱
尚佳健
ZHOU Qingnan;ZHAO Huanying;CAI Shuang;YANG Ningyan;ZHOU Yan;SHANG Jiajian(.1.Department of Pediatric Dentistry,Capital Medical University,Beijing 100050,China;Ge-nomics Research Platform Core Facilities Center,Capital Medical University,Beijing 100069,China)
出处
《口腔疾病防治》
2023年第4期267-273,共7页
Journal of Prevention and Treatment for Stomatological Diseases
基金
北京市医院管理中心儿科学科协同发展中心专项经费资助项目(XTYB201830)。
关键词
重度低龄儿童龋
全身麻醉
口腔微生态
菌斑微生物群落
微生物多样性
生物失调
菌斑生物膜
龋病管理
severe early childhood caries
dental general anesthesia
oral microecology
plaque microbial com⁃munity
microbiome diversity
dysbiosis
biofilm
caries management
