摘要
To discuss the inhibitory effect of supramolecular azelaic acid(SAZA)and azelaic acid(AZA)on melanin synthesis.The inhibition rate of tyrosinase activity was tested in vitro.Zebrafish embryos were exposed to SAZA and AZA in different proportions.The development of embryo melanin was observed and quantitatively analyzed.The experimental result showed that both AZA and SAZA had inhibitory effect on tyrosinase,with IC_(50) of 8.34 mmol/L for AZA and 6.56 mmol/L for SAZA.Zebrafish embryo is basically normally developed,with no obvious deformity.Both AZA and SAZA can inhibit the production of melanin,and the SAZA has a better effect in the same amount of experiment.In addition,AZA is able to inhibit the tyrosinase activity and melanin production in zebrafish embryos.The SAZA prepared by cyclodextrin inclusion has shown better water solubility and significantly improved compatibility.
