摘要
目的探讨愈风宁心片对偏头痛大鼠模型的镇痛作用及机制。方法SD大鼠随机分成6组:对照组、模型组、苯甲酸利扎曲普坦片(阳性药,0.9 mg·kg^(-1))组和愈风宁心片低、中、高剂量(378、756、1512mg·kg^(-1))组,每天ig给药1次,连续10 d。末次给药后1 h,除对照组外,其余各组大鼠在头颈处sc 10 mg·kg^(-1)硝酸甘油建立偏头痛大鼠模型,观察疼痛行为学指标,试剂盒法检测血清中疼痛相关因子缩胆囊素(CCK)、白细胞介素-1β(IL-1β)、前列腺素E_(2)(PGE)_(2)含量;利用小鼠醋酸扭体实验、小鼠右后足跖部福尔马林致痛实验观察愈风宁心片低、中、高剂量(546、1092、2184 mg·kg^(-1))对小鼠扭体反应和舔足时长的影响。结果偏头痛实验结果表明,与模型组比较,愈风宁心片各剂量组和苯甲酸利扎曲普坦片组的挠头、甩头次数和行为学评分显著降低(P<0.05、0.01);愈风宁心片中、高剂量组血清CCK水平显著降低(P<0.05、0.01),低、中、高剂量组血清PGE、IL-1β水平显著降低(P<0.05、0.01)。醋酸扭体实验结果表明,与模型组比较,愈风宁心片中、高剂量组小鼠扭体次数显著降低(P<0.05、0.01),高剂量组小鼠扭体反应潜伏期显著延长(P<0.01)。福尔马林致痛实验结果表明,与模型组比较,各给药组均能显著缩短小鼠的舔足时长(P<0.01)。结论愈风宁心片对偏头痛模型具有镇痛作用,其机制可能与调节中枢及外周神经系统、改善疼痛相关因子含量有关。
Objective To investigate the analgesic effect and mechanism of Yufeng Ningxin Tablet on migraine in rats. Method SD rats were randomly divided into six groups: control group, model group, rizatriptan benzoate tablet(positive drug, 0.9 mg·kg^(-1))group and Yufeng Ningxin Tablet low, medium and high dose(378, 756, 1 512 mg·kg^(-1)) groups. The rats were given ig once a day for 10 days. One hour after the last administration, except for control group, the rats in the other groups were treated with sc 10 mg·kg^(-1)nitroglycerin in the head and neck to establish the rat model of migraine. The pain behavioral indexes were observed.The contents of pain related factors cholecystokinin(CCK), interleukin-1β(IL-1β), and prostaglandin E_(2)(PGE)_(2) in serum were detected by kit method. The effects of low, medium and high doses of Yufeng Ningxin Tablets(546, 1 092, 2 184 mg·kg^(-1)) on writhing response and licking time of mice were observed by acetic acid writhing test and formalin pain induced by right hind foot in mice. Results The results of migraine experiment showed that compared with the model group, the number of head scratching, head shaking and behavioral scores of each dose group of Yufeng Ningxin tablet and rizatriptan benzoate tablet group were significantly decreased(P < 0.05, 0.01). The serum levels of CCK in Yufeng Ningxin tablets medium and high-dose groups were significantly decreased(P < 0.05, 0.01), and the serum levels of PGEand IL-1β in Yufeng Ningxin Tablet low, medium and high-dose groups were significantly decreased(P < 0.05, 0.01). The results of acetic acid writhing experiment showed that compared with model group, the writhing times of mice in Yufeng Ningxin tablet medium and high-dose group were significantly decreased(P < 0.05,0.01), and the latency of writhing reaction of mice in high-dose group was significantly prolonged(P < 0.01). The results of formalin induced pain test showed that compared with the model group, each administration group could significantly inhibit the licking duration of mice(P < 0.01). Conclusion Yufeng Ningxin Tablet has analgesic effect on migraine model, and its mechanism may be related to regulating central and peripheral nervous system and improving the content of pain-related factors.
作者
于尚玥
范春兰
李依林
裴海鸾
田颖颖
左泽平
赵新月
刘闯
王志斌
YU Shangyue;FAN Chunlan;LI Yilin;PEI Hailuan;TIAN Yingying;ZUO Zeping;ZHAO Xinxue;LIU Chuang;WANG Zhibin(College of traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488,China;Tongrentang Pharmaceutical Co.,Ltd.,Beijing 100071,China)
出处
《药物评价研究》
CAS
2022年第11期2208-2214,共7页
Drug Evaluation Research
基金
国家重点研发计划(2019YFC1711400)。
关键词
愈风宁心片
偏头痛
镇痛
疼痛相关因子
醋酸扭体实验
福尔马林
Yufeng Ningxin Tablet
migraine
analgesic effect
pain related factor
acetic acid torsion test
formalin
