摘要
为了探究褪黑素(Mt)对低温环境下菜豆种子的影响,本研究对菜豆种子进行低温处理(LT)和低温加褪黑素的处理(LT+Mt),并设置常温处理作为对照(CK),对同时期的各项生理指标进行测定。结果表明:Mt可以提高低温胁迫下的种子萌发率,但萌发率要低于对照;同时对渗透调节物质、O^(-)_(2)和MDA、抗氧化酶活性进行测定,发现低温增加了渗透调节物质的含量,当加入Mt后其含量进一步增加;低温处理后O^(-)_(2)和MDA的含量增加,当加入Mt后其含量明显降低,但是要高于对照;低温处理后抗氧化酶活性略低于对照,当加入Mt后其含量显著升高。研究表明外源Mt有效的减轻了低温对种子萌发的损害,从而促进种子萌发过程中抵御低温的能力。
In order to explore the effect of melatonin(Mt)on common bean seeds under chilling stress,the seeds of common bean were treated with chilling stress(LT)and chilling stress with melatonin(LT+Mt),and the normal temperature treatment was set as the control(CK).The physiological indexes of seeds of the same period were measured.The results showed that Mt could improve the germination rate of seeds under chilling stress,but the germination rate was lower than that of CK.The content of osmoregulatory substances,O^(-)_(2)and MDA and the activities of antioxidant enzymes were measured.It was found that the content of osmoregulatory substances increased under chilling stress,and the content further increased when Mt was added.The content of O^(-)_(2)and MDA increased after chilling stress,while the content of MDA decreased significantly after Mt addition,but was higher than that of CK.The activities of antioxidant enzymes after low temperature treatment were slightly lower than those of CK,and the content of antioxidant enzymes was significantly increased when Mt was added.To sum up,exogenous Mt could effectively reduce the damage of chilling stress on seed germination,thus promoting the ability of seeds to resist chilling stress during germination.
作者
杨晓旭
李梦娣
刘大军
冯国军
刘畅
YANG Xiaoxu;LI Mengdi;LIU Dajun;FENG Guojun;LIU Chang(College of Modern Agriculture and Ecological Environment,Heilongjiang University,Harbin 150080;College of Life Sciences,Heilongjiang University,Harbin 150080)
出处
《中国农学通报》
2022年第33期34-38,共5页
Chinese Agricultural Science Bulletin
基金
黑龙江省高校基本科研业务费“菜豆光周期开花调控相关基因的筛选与验证”(2020-KYYWF-1027),“菜豆叶片黄转绿突变体ytg的定位与克隆”(2020-KYYWF-1026)。
关键词
菜豆
褪黑素
种子
低温胁迫
common bean
melatonin
seed
chilling stress
