摘要
目的探讨T细胞受体γ位点反义RNA 1(TRG-AS1)、人抗原R(HuR)、三元基序家族蛋白21(TRIM21)、缺氧诱导因子-1α(HIF-1α)在结直肠癌组织中的表达及临床意义。方法收集103例结直肠癌患者的结直肠癌组织和癌旁组织,采用免疫组织化学染色法检测HuR、TRIM21、HIF-1α表达情况,采用实时定量逆转录聚合酶链反应(qRT-PCR)检测TRG-AS1相对表达量。比较结直肠癌组织和癌旁组织中TRG-AS1、HuR、TRIM21、HIF-1α的表达情况,分析TRG-AS1、HuR、TRIM21、HIF-1α表达情况与结直肠癌患者临床特征和预后的关系。结果结直肠癌组织中TRG-AS1相对表达量及HuR、HIF-1α阳性率均明显高于癌旁组织,TRIM21阳性率明显低于癌旁组织,差异均有统计学意义(P﹤0.01)。不同分化程度、淋巴结转移情况、血管侵犯情况结直肠癌患者结直肠癌组织中TRG-AS1相对表达量比较,差异均有统计学意义(P﹤0.05)。不同浸润深度、TNM分期、淋巴结转移情况结直肠癌患者结直肠癌组织中HuR、TRIM21、HIF-1α阳性率比较,差异均有统计学意义(P﹤0.05)。至随访结束,TRG-AS1低表达患者的累积生存率为55.6%,高于TRG-AS1高表达患者的31.5%(P﹤0.05);HuR阴性患者的累积生存率为56.9%,高于HuR阳性患者的37.0%(P﹤0.05);TRIM21阴性患者的累积生存率为22.4%,低于TRIM21阳性患者的59.0%(P﹤0.05);HIF-1α阴性患者的累积生存率为72.0%,高于HIF-1α阳性患者的36.6%(P﹤0.05)。结论结直肠癌组织中TRG-AS1、HIF-1α及HuR表达均升高,TRIM21表达降低,TRG-AS1、HuR、TRIM21、HIF-1α表达情况与结直肠癌侵袭、转移及患者预后密切相关,对评估结直肠癌患者病情及预后具有一定的临床价值。
Objective To investigate the expression and clinical significance of T cell receptor gamma locus antisense RNA 1(TRG-AS1),human antigen R(HuR),tripartite motif-containing protein 21(TRIM21)and hypoxia inducible factor-1α(HIF-1α)in colorectal cancer tissues.Method The colorectal cancer tissues and paracancerous tissues of103 colorectal cancer patients were collected.The expression of HuR,TRIM21,and HIF-1αwere detected by immunohistochemical staining.The relative expression of TRG-AS1 was detected by real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR).The expression of TRG-AS1,HuR,TRIM21,and HIF-1αin colorectal cancer tissues and paracancerous tissues were compared.The relationship between the expression of TRG-AS1,HuR,TRIM21,HIF-1αand the clinical characteristics and prognosis of colorectal cancer patients were analyzed.Result The relative expression of TRG-AS1 and the positive rate of HuR and HIF-1αin colorectal cancer tissues were significantly higher than those in paracancerous tissues,the positive rate of TRIM21 was significantly lower than that of paracancerous tissues,and the difference was statistically significant(P<0.01).There were statistically significant differences in the relative expression of TRG-AS1 in colorectal cancer tissues of colorectal cancer patients with different differentiation degrees,lymph node metastasis,and vascular invasion(P<0.05).There were statistically significant differences in the positive rate of HuR,TRIM21,and HIF-1αin colorectal cancer tissues of colorectal cancer patients with different invasion depths,TNM stages,and lymph node metastasis(P<0.05).At the end of follow-up,the cumulative survival rate of patients with low expression of TRG-AS1 was 55.6%,higher than 31.5%of patients with high expression of TRG-AS1(P<0.05);the cumulative survival rate of HuR-negative patients was 56.9%,higher than 37.0%of HuR-positive patients(P<0.05);the cumulative survival rate of TRIM21-negative patients was 22.4%,lower than 59.0%of TRIM21-positive patients(P<0.05);the cumulative survival rate of HIF-1α-negative patients was 72.0%,higher than 36.6%of HIF-1α-positive patients(P<0.05).Conclusion The expression of TRG-AS1,HIF-1α,and HuR were all increased,while the expression of TRIM21 was decreased in colorectal cancer tissues.The expression of TRG-AS1,HuR,TRIM21,and HIF-1αare closely related to the invasion,metastasis,and prognosis of colorectal cancer patients.These indicators have certain clinical value in evaluating the condition and prognosis of patients with colorectal cancer.
作者
柴小兵
褚菲菲
肖兴国
吴慧丽
CHAI Xiaobing;CHU Feifei;XIAO Xingguo;WU Huili(Department of Gastroenterology,Zhengzhou Central Hospital Affiliated to Zhengzhou University,Medical Key Laboratory for Diagnosis and Treatment of Colorectal Cancer in He’nan Province,Zhengzhou Key Laboratory for Diagnosis,Treatment and Research of Colorectal Cancer,Zhengzhou 450007,He’nan,China)
出处
《癌症进展》
2022年第23期2409-2414,共6页
Oncology Progress
基金
河南省医学科技攻关计划项目(LHGJ20210763)。
关键词
T细胞受体γ位点反义RNA
1
人抗原R
三元基序家族蛋白21
缺氧诱导因子-1Α
结直肠癌
临床特征
预后
T cell receptor gamma locus antisense RNA 1
human antigen R
tripartite motif-containing protein 21
hypoxia inducible factor-1α
colorectal cancer
clinical characteristic
prognosis
