摘要
目的 对不同产地枇杷叶的质量进行综合评价。方法 以不同产地的30份枇杷叶为样品,采用热浸法测定其醇溶性浸出物含量,采用紫外分光光度法分别测定其总黄酮含量和总三萜酸含量,采用高效液相色谱法测定其5个三萜酸类成分(野鸦椿酸、山楂酸、科罗索酸、齐墩果酸及熊果酸)的含量。采用熵权逼近理想排序法(TOPSIS)综合评价不同产地枇杷叶的质量;运用SPSS22.0软件对枇杷叶片重、综合评价值、醇溶性浸出物含量、总黄酮含量、总三萜酸含量及5个三萜酸类成分含量进行双变量相关性分析。结果 30份不同产地枇杷叶中醇溶性浸出物含量为(24.56±0.08)%~(34.85±0.13)%,总黄酮含量为(4.69±0.11)~(14.23±0.27) mg/g,总三萜酸含量为(27.58±0.59)~(63.95±1.27) mg/g,野鸦椿酸、山楂酸、科罗索酸、齐墩果酸、熊果酸的含量分别为(0.728±0.011)~(6.064±0.063)、(0.526±0.013)~(3.245±0.022)、(1.222±0.025)~(8.807±0.094)、(0.856±0.021)~(2.931±0.075)、(4.704±0.087)~(11.806±0.283) mg/g;熵权TOPSIS法分析结果显示,综合评价值排名前3位的样品依次为S14(福建漳州云霄县火田镇产)、S19(广西钦州钦南区产)及S29(安徽亳州涡阳县产)。枇杷叶的综合评价值与野鸦椿酸、山楂酸、科罗索酸、齐墩果酸和熊果酸这5个三萜酸类成分的含量均呈正相关(P<0.01),且枇杷叶片重与综合评价值呈正相关(P<0.01)。结论 不同产地枇杷叶质量存在较大差异,其中以福建漳州云霄县火田镇产、广西钦州钦南区产和安徽亳州涡阳县产枇杷叶的质量相对较优;枇杷叶片重与其品质呈正相关。
OBJECTIVE To comprehensively evaluate the quality of Eriobotrya japonica leaves from different producing areas.METHODS The contents of alcohol-soluble extracts were determined by hot-dipping method using 30 batches of E. japonica leaves from different producing areas as samples. The contents of total flavonoids and total triterpene acids were determined by ultraviolet spectrophotometry. The contents of five kinds of triterpenic acids(euscaphic acid,crataegolic acid,corosolic acid,oleanolic acid and ursolic acid) were determined by HPLC. The quality of E. japonica leaves from different producing areas was comprehensively evaluated by using entropy weight technique for order preference by similarity to an ideal solution(TOPSIS). The bivariate correlation analysis of E. japonica leaves was conducted by SPSS 22.0 software in terms of weight, comprehensive evaluation value, the content of alcohol-soluble extract, the contents of total flavonoids, total triterpene acids and five triterpenic acids.RESULTS The contents of alcohol-soluble extract in 30 batches of E. japonica leaves were(24.56±0.08)%-(34.85±0.13)%;the contents of total flavonoids were(4.69±0.11)-(14.23±0.27) mg/g;the contents of total triterpene acid were(27.58±0.59)-(63.95±1.27) mg/g;the contents of euscaphic acid, crataegolic acid, corosolic acid, oleanolic acid and ursolic acid were(0.728±0.011)-(6.064±0.063),(0.526±0.013)-(3.245±0.022),(1.222±0.025)-(8.807±0.094),(0.856±0.021)-(2.931±0.075), (4.704±0.087)-(11.806±0.283) mg/g, respectively. The analysis result of entropy weight TOPSIS method showed that the top three samples with comprehensive evaluation values were S14(Huotian Town, Yunxiao County, Zhangzhou, Fujian), S19(Qinnan District, Qinzhou, Guangxi) and S29(Guoyang County, Bozhou, Anhui). Comprehensive evaluation of E. japonica leaves was positively correlated with the contents of five kinds of triterpenic acids, such as euscaphic acid, crataegolic acid, corosolic acid, oleanolic acid and ursolic acid(P<0.01). The weight of E. japonica leaves was positively correlated with the comprehensive evaluation value(P<0.01). CONCLUSIONS The qualities of E. japonica leaves from different producing areas are very different. Among them, the qualities of E. japonica leaves from Huotian Town, Yunxiao County, Zhangzhou of Fujian, Qinzhou Qinnan District of Guangxi, and Bozhou Guoyang County of Anhui are relatively better. The weight of E. japonica leaves is positively correlated with their quality.
作者
高伟城
王小平
肖晓琳
施义炜
林少芬
GAO Weicheng;WANG Xiaoping;XIAO Xiaolin;SHI Yiwei;LIN Shaofen(Dept.of Pharmacy,Zhangzhou Health Vocational College,Fujian Zhangzhou 363000,China;Dept.of Pharmacy,Zhangzhou Municipal Hospital of Fujian Province,Fujian Zhangzhou 363000,China;Zhangzhou Jushantang Pharmaceutical Co.,Ltd.,Fujian Zhangzhou 363000,China)
出处
《中国药房》
CAS
北大核心
2023年第1期72-77,共6页
China Pharmacy
基金
全国中药资源普查项目(No.财社[2019]39号)
漳州市市级个人科技特派员资助项目(No.20210187)
漳州卫生职业学院科技创新团队培育计划项目(No.Kjcx-5)。
关键词
枇杷叶
三萜酸类成分
总黄酮
熵权逼近理想排序法
相关性分析
产地
Eriobotrya japonica leaves
triterpene acids
total flavonoids
entropy weight TOPSIS method
correlation analysis
producing areas
