摘要
【目的】明确沙田柠檬炭疽病的病原菌种类,为沙田柠檬炭疽病的田间防治提供指导。【方法】对采集的炭疽病样本进行分离纯化培养,获得ST2-1、ST2-2、ST2-3、ST3-1、ST3-2、ST3-3、ST4-1、ST4-2和ST4-3等9株沙田柠檬炭疽病病原菌。分别采用针刺和喷洒接种的方法测定上述菌株的致病性,并分别克隆其核糖体内转录间隔区(ITS)、β微管蛋白(TUB2)、钙调蛋白(CAL)、肌动蛋白(ACT)、核糖体大亚基(LSU)、甘油醛-3-磷酸脱氢酶(GAPDH)和几丁质合成酶1(CHS1)基因部分片段进行多基因联合鉴定。【结果】ST2-1、ST2-2、ST2-3、ST3-1、ST3-2、ST3-3、ST4-1、ST4-2和ST4-3均具有较强致病性,其中ST2-1、ST2-2和ST2-3的生物学特征和进化树表明它们属于暹罗刺盘孢菌(Colletotrichum siamense),ST3-1、ST3-2和ST3-3属于兰花刺盘孢菌(Colletotrichum cliviicola),ST4-1、ST4-2和ST4-3属于果生刺盘孢菌(Colletotrichum fructicola)。【结论】综合形态学特征和分子生物学鉴定结果,引起沙田柠檬炭疽病的病原菌有暹罗刺盘孢菌、兰花刺盘孢菌和果生刺盘孢菌,其中暹罗刺盘孢菌仅分离自沙田柠檬发病的果实,兰花刺盘孢菌和果生刺盘孢菌分离自沙田柠檬发病的叶片,其中兰花刺盘孢菌能在柑橘类植物上引起炭疽病为国际上首次报道。
【Objective】The study aimed to clarify the types of pathogens causing anthracnose on Shatian lemon, with a view to providing guidance for the prevention and control of anthracnose on Shatian lemon in field.【Method】The collected anthracnose samples were isolated and purified, and 9 strains of the pathogens ST2-1, ST2-2, ST2-3, ST3-1, ST3-2,ST3-3, ST4-1, ST4-1, ST4-2,and ST4-3 of anthracnose on Shatian lemon were obtained. Then puncturing and spraying inoculation methods were used to test the pathogenicity of the above strains, and their ITS, TUB2, ACT, LSU, GAPDH and CAL fragments were cloned for multi-gene joint identification.【Result】ST2-1, ST2-2, ST2-3, ST3-1, ST3-2, ST3-3, ST4-1,ST4-2 and ST4-3 are all highly pathogenic. The biological characteristics and phylogenetic tree indicated that ST2-1, ST2-2 and ST2-3 belonged to Colletotrichum siamense, ST3-1, ST3-2 and ST3-3 belonged to Colletotrichum cliviicola, ST4-1, ST4-2 and ST4-3 belonged to Colletotrichum fructicola.【Conclusion】Based on the morphological characteristics and molecular biological identification results, the pathogens causing anthracnose on Shatian lemon in Guangzhou were C. siamense,C. cliviicola and C. fructicola, in which C. siamese was isolated from the diseased fruit of Shatian lemon, C. cliviicola and C.fructicola were isolated from the diseased leaves of Shatian lemon, and it was the first report in the world that C. cliviicola could cause anthracnose on citrus plants.
作者
崔一平
彭埃天
宋晓兵
冯冠杰
黄峰
陈霞
凌金锋
程保平
CUI Yiping;PENG Aitian;SONG Xiaobing;FENG Guanjie;HUANG Feng;CHEN Xia;LING Jinfeng;CHENG Baoping(Institute of Plant Protection,Guangdong Academy of Agricultural Sciences/Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China,Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of High Technology for Plant Protection,Guangzhou 510640,China;Shatian Lemon Agricultural Products Cooperative of Baiyun District,Guangzhou 510640,China)
出处
《广东农业科学》
CAS
2022年第12期99-109,共11页
Guangdong Agricultural Sciences
基金
广东省现代农业产业技术体系创新团队项目(2022KJ108)
广州市农村科技特派员项目(GZKTP202039)。
关键词
沙田柠檬
广州市
炭疽病
果生刺盘孢菌
兰花刺盘孢
暹罗刺盘孢菌
Shatian lemon
Guangzhou City
anthracnose
Colletotrichum fructicola
Colletotrichum cliviicola
Colletotrichum siamense
