摘要
目的基于NF-κB/STAT3信号通路探究防风多糖对过敏性鼻炎大鼠行为学、AQP5及鼻粘膜组织的影响。方法选取40只SPF级SD雄性大鼠,随机分为正常(N)组,模型(M)组,糠酸莫米松(F)组,防风多糖(W)组,每组10只,采用卵清蛋白致敏法建立过敏性鼻炎模型,N组不建立该模型,建模成功后,对F组给予1喷/侧的糠酸莫米松喷鼻治疗,对W组灌胃600㎎/㎏的防风多糖,N组、M组同期给与灌胃同体积生理盐水,观察大鼠一般情况并对其行为学进行评分,HE染色法检测鼻黏膜组织病理形态,免疫组化法检测鼻黏膜组织中AQP5表达,免疫印迹法检测鼻黏膜组织中NF-κB/STAT3信号通路相关蛋白表达。结果实验开始前各组大鼠均活动饮食正常且精神状态良好,未见流涕、喷嚏、挠鼻等症状,造模完成后,可见大鼠精神状态明显变差,进食及活动明显减少,且可见被毛凌乱无光泽,出现常见抓鼻动作,喷嚏频发,大量鼻分泌物流出鼻前孔,给药后,大鼠纳食摄水都逐渐恢复正常,精神状态良好,喷嚏、流涕、挠鼻等症状都较治疗前有明显缓解,均无脏器损伤,但各组大鼠体重无明显变化(P>0.05);与N组相比,M组行为学评分显著升高(P<0.05),与M组比较,F、W两组行为学评分显著降低(P<0.05),且W组比F组降低显著(P<0.05);N组大鼠鼻黏膜组织结构完整、平滑且排列整齐,腺体大小正常,未见明显上皮坏死脱落、炎性细胞浸润及明显血管扩张或充血现象,M组鼻黏膜上皮结构紊乱且不完整,可见鼻粘膜上皮细胞破坏变性,出现纤毛坏死甚至脱落现象,可见明显腺体增生、肿胀、出血及炎性细胞浸润,与M组相比,F组、W组病理状态明显改善,炎性细胞明显减少;与N组比较,M组鼻黏膜组织中AQP5蛋白表达显著降低(P<0.05),与M组比较,F组、W组鼻黏膜组织中AQP5蛋白表达显著升高(P<0.05),且W组比F组升高显著(P<0.05);与N组比较,M组鼻黏膜组织中NF-κB、P-NF-κB、STAT3、P-STAT3蛋白表达显著升高(P<0.05),与M组比较,F组、W组鼻黏膜组织中NF-κB、P-NF-κB、STAT3、P-STAT3蛋白表达显著降低(P<0.05),且W组比F组变化显著(P<0.05),而B组与W组相比无明显差异(P>0.05),O组比B组降低明显(P<0.05)。结论防风多糖可有效改善过敏性鼻炎大鼠行为学及鼻黏膜病理形态,并可显著促进AQP5表达,其机制可能与抑制NF-κB/STAT3信号通路有关。
Objective To explore the effects of polysaccharides from radix kostyniae japonicae on behavior,AQP5 and nasal mucosa tissues in rats with allergic rhinitis based on NF-κB/STAT3 signaling pathway.Methods 40 SPF SD male rats were randomly divided into normal group(N),model group(M),furoic acid mo betamethasone(group F),wind polysaccharide(W)group,each group of 10.Ovalbumin sensitization method was adopted to establish the model of allergic rhinitis.N group is not set up the model.After the success of the modeling,the group F give 1 spray/side furoic acid mo betamethasone nasal spray treatment.The polysaccharide of 600mg/kg was gavaged to group W,and the same volume of normal saline was gavaged to group N and GROUP M at the same time.The general situation of rats was observed and their behavioral scores were scored.The pathological morphology of nasal mucosa tissue was detected by HE staining,and the expression of AQP5 in nasal mucosa tissue was detected by immunohistochemistry.The expression of NF-κB/STAT3 signaling pathway in nasal mucosa was detected by western blotting.Results Before the experiment,all the rats in each group had normal diet and good mental state,and no symptoms such as runny nose,sneezing and nose scratching were observed.After the modeling was completed,the mental state of the rats was significantly deteriorated,eating and activities were significantly reduced,and the coat was disheveled and lustrous,with common nose scratching,frequent sneezing,and a large amount of nasal secretions flowing out of the anterior nasal foramina.After the treatment,the rat feed water are taken gradually returned to normal,good mental state,sneezing,runny nose,scratching nose symptoms before the treatment have obvious relief,no injury,but each rat no obvious change in body weight(P>0.05).Compared with the N group,a significant rise in M group behavior score(P<0.05).Compared with M group,F and W groups of behavior score decreased significantly(P<0.05),and W group reduced significantly than group F(P<0.05).The nasal mucosa structure of rats in group N was complete,smooth and orderly,and the size of glands was normal.No obvious epithelial necrosis and exfoliation,inflammatory cell infiltration,and obvious vasodilation or congestion were observed.The epithelial structure of the nasal mucosa in group M was disordered and incomplete,with destruction and degeneration of the epithelial cells of the nasal mucosa,ciliary necrosis and even shedding,and obvious glandular hyperplasia,swelling,bleeding and inflammatory cell infiltration.Compared with group M,the pathological status of group F and group W was significantly improved,and the inflammatory cells were significantly reduced.Compared with N group,group M AQP5 protein expression significantly reduced in nasal mucosa(P<0.05).Compared with group M,F,W group AQP5 protein expression was significantly increased in nasal mucosa(P<0.05),and W group increased significantly than group F(P<0.05).Compared with N group,group M NF-kappa B predominate in the nasal mucosa,P-NF-kappa B predominate,STAT3,P-STAT3 protein expression was significantly elevated(P<0.05).Compared with group M,F,W group NF-kappa B predominate in the nasal mucosa,P-NF-kappa B predominate,STAT3,P-STAT3 protein expression significantly reduced(P<0.05),and W group than in group F changes significantly(P<0.05),but no obvious difference was found between group B compared with group W(P>0.05),group O were significantly lower than group B(P<0.05).Conclusion Polysaccharides from P.simokata can effectively improve the behavior and pathological morphology of nasal mucosa in rats with allergic rhinitis,and significantly promote the expression of AQP5,which may be related to the inhibition of NF-κB/STAT3 signaling pathway.
作者
任俊秀
韩燕
张珍霞
任安娜
陈元平
李欣
REN Junxiu;HAN Yan;ZHANG Zhengxia;REN Anna;CHEN Yuanping;LI Xin(Linfen Central Hospital,Linfen 041000,Shanxi,China;Shanxi Provincial People's Hospital,Taiyuan 030012,China)
出处
《西部医学》
2023年第1期39-45,共7页
Medical Journal of West China
基金
山西省基础研究项目(2015021182)。
