摘要
【目的】探究‘凤丹’种子休眠解除过程中几种抑制物质含量的变化规律及其与‘凤丹’种子休眠的关系,旨在为种子休眠机理研究以及更好地解除休眠、促进种子萌发提供理论依据。【方法】以‘凤丹’种子为材料,采用系统溶剂分离法和气相色谱-质谱联用(GC-MS)技术,筛选出可能对‘凤丹’种子发芽有影响的内源抑制物,测定了其抑制中浓度及其在休眠解除不同阶段的活性与含量。【结果】层积不同阶段‘凤丹’种子的浸提液,对白菜种子发芽有不同程度的抑制作用:未层积(Ⅰ阶段)的‘凤丹’种子的浸提液仅能使20.7%的白菜种子发芽,较对照的白菜种子发芽率下降了77.4%;当‘凤丹’种子低温层积至胚根突破种皮(Ⅲ阶段)时,其浸提液使白菜种子的发芽率降为46.3%,较对照下降了49.5%;‘凤丹’种子层积至真叶出现的Ⅴ阶段时,白菜种子的发芽率达到88.0%,较对照略有下降,但差异不显著。油酸对白菜种子发芽无抑制作用,邻苯二酚、亚油酸、棕榈酸、硬脂酸这4种标准品对白菜种子发芽均有一定程度的影响,按照抑制中浓度由低到高的顺序为:邻苯二酚<亚油酸<棕榈酸<硬脂酸,其抑制中浓度分别为12.13、137.41、460.26、972.75 mg/L。‘凤丹’种子层积至Ⅲ阶段时,内源抑制物质按照下降幅度由高到低的变化顺序为:亚油酸>硬脂酸>棕榈酸。层积至Ⅴ阶段时,邻苯二酚的含量降为0;棕榈酸、硬脂酸的含量均显著降低,较其在Ⅲ阶段的含量分别下降了25.1%、32.0%;亚油酸的含量略有上升。【结论】邻苯二酚、亚油酸、棕榈酸、硬脂酸这4种物质对‘凤丹’种子的萌发均有一定程度的影响,但在种子层积的不同阶段,起抑制作用的物质不相同:亚油酸、硬脂酸、棕榈酸这3种物质对胚根突破种皮有一定程度的影响;棕榈酸、硬脂酸、邻苯二酚对上胚轴的伸长均有较强的抑制作用。
【Objective】This paper explored the changes of the content of several inhibitors in the‘Feng Dan’seeds during the process of dormancy break and its relationship with the dormancy of‘Feng Dan’seeds,so as to provide a theoretical basis for the study of the mechanism of seed dormancy and better break seed dormancy and promote seed germination.【Method】Using the seeds of‘Feng Dan’as materials,the endogenous inhibitors that may affect the germination of‘Feng Dan’seeds were screened by systematic solvent separation and gas chromatography-mass spectrometry(GC-MS).The concentration of the endogenous inhibitors and the changes of the activity and content of these endogenous inhibitors during different stages of dormancy release were determined.【Result】The extracts of P.ostii‘Feng Dan’seeds at different stratification stages had different degrees of inhibition on the germination of cabbage seeds:when treated with the extracts of‘Feng Dan’seeds without stratification(stageⅠ),the germination percentage of cabbage seeds was only 20.7%,which decreased by 77.4%compared with the control.When the seeds of P.ostii‘Feng Dan’stratified at low temperature until the radicle broke through the seed coat(stageⅢ),the germination percentage of cabbage seeds decreased to 46.3%,which was 49.5%lower than the control.In the fifth stage of stratification,the leaves appeared and the germination percentage of cabbage seeds reached 88.0%,which was slightly lower than that of the control,but the difference was not significant.Oleic acid had no inhibitory effect on the germination of cabbage seeds,while catechol,linoleic acid,palmitic acid and stearic acid had certain effects on the germination of cabbage seeds.According to the effective inhibition concentration from low to high:the order was catechol<linoleic acid<palmitic acid<stearic acid,and their medium inhibitory concentrations were 12.13 mg/L,137.41 mg/L,460.26 mg/L,and 972.75 mg/L respectively.When the seed stratification of P.ostii‘Feng Dan’reached stageⅢ,the descending order of endogenous inhibitors from high to low was:linoleic acid>stearic acid>palmitic acid.At stage V,the content of catechol decreased to 0;the content of palmitic acid and stearic acid decreased significantly by 25.1%and 32.0%respectively compared with stageⅢ;the content of linoleic acid increased slightly.【Conclusion】The catechol,linoleic acid,palmitic acid,and stearic acid had a certain influence on the germination of P.ostii‘Feng Dan’seeds,but the acting inhibitory substances were different at different stages:linoleic acid,stearic acid,and palmitic acid had certain effects on the breakthrough of the radicle of P.ostii‘Feng Dan’seeds;the palmitic acid,stearic acid and catechol had a strong inhibitory effect on the elongation of epicotyl.
作者
李强
孙海燕
朱铭玮
翟金庭
李淑娴
LI Qiang;SUN Haiyan;ZHU Mingwei;ZHAI Jinting;LI Shuxian(Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing,Jiangsu 210037,China;Yancheng Forest Farm,Yancheng 224136,Jiangsu 210037,China)
出处
《经济林研究》
北大核心
2022年第4期81-89,共9页
Non-wood Forest Research
基金
江苏省林业科技创新与推广项目(LYKJ〔2019〕38)
南京市高淳区科技成果入乡转化项目(2021012)
江苏高校优势学科建设资金资助项目(PAPD)。
