摘要
目的探讨丝氨酸蛋白酶抑制剂A1(SERPINA1)在肝癌组织的表达及临床意义。方法选取行手术切除的78例肝癌病例,采用实时荧光定量PCR(RT-PCR)和Western blot检测肝癌组织和癌旁组织SERPINA1 mRNA和蛋白表达;免疫组织化学检测肝癌组织中SERPINA1表达。根据SERPINA1表达将肝癌患者分为SERPINA1高表达组和SERPINA1低表达组,分析SERPINA1表达与肝癌转移和预后的关系。采用RNA干扰技术干扰肝癌HepG2细胞中的SERPINA1表达,Transwell侵袭实验检测SERPINA1对肝癌细胞侵袭能力的影响,Western blot检测上皮-间质转化(EMT)相关蛋白E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)和金属基质蛋白酶9(MMP-9)表达的变化。结果患者肝癌组织中SERPINA1 mRNA和蛋白表达明显高于癌旁组织(t=4.872、5.811,P<0.05)。肝癌组织中SERPINA1阳性表达率明显高于癌旁组织(χ^(2)=5.03,P<0.05),肝癌组织中SERPINA1阳性表达与TNM分期、病理分级和血管侵犯有关(P<0.05)。SERPINA1高表达组肝癌患者5年累积生存率低于SERPINA1低表达组(χ^(2)=5.899,P<0.05),5年累积复发率高于SERPINA1低表达组(χ^(2)=7.305,P<0.01)。HepG2细胞沉默SERPINA1表达后,穿过Transwell小室基底膜的细胞数较对照组和Mock组减少,上皮标志物E-cadherin表达升高,间质标志物N-cadherin和MMP-9表达降低。结论SERPINA1表达与肝癌的侵袭转移和预后相关,其可能是预防肝癌转移和早期干预的新靶点。
Objective To investigate the expression and clinical significance of serine protease inhibitor A1(SERPINA1)in hepatocellular carcinoma(HCC)tissues.Methods Seventy-eight cases of HCC excision were selected.The expression of SERPINA1 mRNA and protein in carcinoma and corresponding paracancerous tissues was detected by using real-time PCR and Western blot respectively.The immunohistochemical analysis was used to detect the expression of SERPINA1 in HCC tissues,and the HCC patients were divided into the SERPINA1 high expression group and SERPINA1 low expression group according to the expression level of SERPINA1 in HCC tissues.The relationship between the expressions of SERPINA1 with metastasis and clinical characteristics of HCC was analyzed.The RNA interference technique was adopted to interfere the SERPINA1 expression in HCC hepG2 cells.The Transwell invasion assay was used to detect the effect of SERPINA1 on the invasion ability of HCC cells.The expression of epithelial-mesenehymaltransition(EMT)associated markers E-cadherin,N-cadherin and MMP-9 in HCC cells was detected by using Western blot analysis.Results The mRNA and protein expressions of SERPINA1 in HCC tissues were significantly higher than that in paracancerous tissues(t=4.872,5.811,P<0.05).The positive expression rate of SERPINA1 in HCC tissues was significantly higher than that in paracancerous tissues(χ^(2)=5.03,P<0.05).The positive expression of SERPINA1 was related with the TNM type,pathological classification and vascular invasion(P<0.05).The 5-year accumulate survival rate of the HCC patients in the high SERPINA1 expression group was lower than that in the low expression group(χ^(2)=5.899,P<0.05).The 5-year accumulate tumor recurrence rate in the SERPINA1 high expression group was higher than that in the SERPINA1 low expression group(χ^(2)=7.305,P<0.01).After SERPINA1 expression was silenced in HepG2 cells,the number of cells passing through the basement membrane of Transwell chamber was reduced compared with the control group and Mock group,the expression of epithelial marker E-cadherin in the SERPINA1 knockdown group was increased,while the expression of N-cadherin and MMP-9 was decreased.Conclusion The expression of SERPINA1 is related with invasion,metastasis and prognosis of HCC,which may be the new target spot for preventing HCC metastasis and conducting early intervention.
作者
彭契六
朱春玲
张磊
韦尚谋
罗佳佳
甘丽英
张智
韦邦宁
PENG Qiliu;ZHU Chunling;ZHANG Lei;WEI Shangmou;LUO Jiajia;GAN Liying;ZHANG Zhi;WEI Bangning(Department of Clinical Laboratory,Guangxi International Zhuang Medicine Hospital,Nanning,Guangxi 530201,China;Department of Hepatobiliary Surgery,Nanning Municipal First People’s Hospital,Nanning,Guangxi 530022,China;Department of Hepatobiliary Surgery,Guangxi International Zhuang Medicine Hospital,Nanning,Guangxi 530201,China)
出处
《重庆医学》
CAS
2023年第1期77-82,共6页
Chongqing medicine
基金
广西自然科学基金面上项目(2017JJA10583)
广西国际壮医医院优势学科项目(院字[2021]33号)
2019年广西中医药大学引进博士科研启动基金项目(2019BS042)。
关键词
肝癌
丝氨酸蛋白酶抑制剂A1
上皮-间质转化
侵袭
预后
hepatocellular carcinoma
serine protease inhibitor A1
epithelial-mesenehymal transition
metastasis
prognosis
