摘要
目的探讨南京市乙肝病人乙型肝炎病毒(HBV)-P区突变特点与肝癌易感性的相关性。方法选取2018年1月至2020年12月南京市第二医院乙肝病人1100例,所有病人均采用聚合酶链反应(PCR)检测HBV基因分型及其P区突变位点情况,结合病理检查明确肝癌的发生情况,分析HBV-P区突变特点与肝癌易感性的相关性。结果结合病理检查结果,纳入的1100例的乙肝病人中,肝癌发生率为2.18%(24/1100);肝癌组HBV-P区突变率高于乙肝组,差异有统计学意义(P<0.05);进一步分析HBV-P区突变特点发现,肝癌组rt204、rt180、rt250位点突变率高于乙肝组,差异有统计学意义(P<0.05);logistic回归分析结果显示,rt204、rt180、rt250位点突变是乙肝病人发生肝癌易感性的独立影响因素(P<0.05)。结论南京市乙肝病人HBVP区突变特点与肝癌易感性有关,其中rt204、rt180、rt250位点突变可能易导致肝癌发生。
Objective To investigate the correlation between hepatitis B virus(HBV)-P zone mutation and hepatocellular carcinoma(HCC)susceptibility in patients with hepatitis B in Nanjing.Methods A total of 1100 patients with Hepatitis B from January 2018 to December 2020 in Nanjing Second Hospital were selected.All patients were tested for HBV genotyping and mutation sites in P zone by Polymerase chain reaction(PCR).The occurrence of liver cancer was determined according to pathological examination results,and the correlation between HBV-P zone mutation characteristics and liver cancer susceptibility was analyzed.Results According to the results of pathological examination,the incidence of liver cancer was 2.18%(24/1100)in 1100 patients with hepatitis B.The mutation rate of HBV-P region in liver cancer group was higher than that in hepatitis B group,and the difference was statistically significant(P<0.05);Further analysis of the mutation characteristics of HBV-P region showed that the mutation rates of rt204,rt180 and rt250 sites in liver cancer group were higher than those in hepatitis B group,and the difference was statistically significant(P<0.05);Logistic regression analysis showed that rt204,rt180 and rt250 mutations were independent factors influencing the susceptibility of patients with Hepatitis B to HCC(P<0.05).Conclusion The mutation characteristics of HBV-P region in Nanjing patients are related to the susceptibility to liver cancer,among which the mutation of rt204,rt180 and rt250 sites may easily lead to the occurrence of liver cancer.
作者
徐露露
张琴
时瑞
金晓燕
孙梅
XU Lulu;ZHANG Qin;SHI Rui;JIN Xiaoyan;SUN Mei(Molecular and Genetics Diagnosis Laboratory,Nanjing Hospital Affiliated to Nanjing University of Traditional Chinese Medicine,Nanjing Second Hospital,Nanjing,Jiangsu 210003,China)
出处
《安徽医药》
CAS
2023年第2期277-280,共4页
Anhui Medical and Pharmaceutical Journal
基金
江苏省卫生健康委员会科研项目(LGY2020015)。
关键词
乙型肝炎病毒
P区
突变
肝癌
易感性
Hepatitis B virus
P region
Mutation
Liver cancer
Susceptibility
