摘要
目的:探究黑果腺肋花楸果实(Aronia melanocarpa Fruit,AMF)的降血糖活性和对脂代谢的影响。方法:采用链脲佐菌素(streptozocin,STZ)法建立Ⅱ型糖尿病(type 2 diabetes mellitus,T2DM)小鼠模型。将小鼠随机分为7组:模型组、盐酸二甲双胍组(40 mg/kg·bw)、AMF总黄酮组(75、150、300 mg/kg·bw)、AMF果汁组(10 mL/kg·bw)及空白对照组。检测各组对T2DM小鼠的生理指标、饮水量、采食量、脏器指数、空腹血糖值的影响,测定血清中甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)、丙二醛(MDA)、超氧化物岐化酶(SOD)含量,并对小鼠肝脏和胰腺组织进行病理学观察。结果:AMF总黄酮组和果汁组均对STZ诱导的T2DM小鼠具有显著的降血糖作用(P<0.05),能降低其脏器指数和TG、TC、LDL-C、HDL-C、MDA、SOD水平(P<0.05),修复小鼠胰腺和肝脏组织,AMF总黄酮组能显著改善T2DM小鼠“三多一少”症状(P<0.05)。AMF总黄酮高剂量组的效果最佳。结论:AMF对STZ诱导的T2DM小鼠具有降血糖、降血脂、修复胰腺和肝脏损伤的功效,作用机制可能与其调节氧化应激相关。
Objective:To investigate the hypoglycemic effect of Aronia melanocarpa fruit(AMF)and its effect on lipid metabolism.Methods:Use streptozotocin(STZ)to induce the type 2 diabetes mellitus(T2DM)mouse model.The models were randomly divided into 7 groups:The model group,the metformin hydrochloride group(40 mg/kg·bw),the AMF total flavonoids groups(75,150,300 mg/kg·bw)and the AMF juice group(10 mL/kg·bw),and the blank control group.This study detected the effects of each group on the physiological indices,water intake,food intake,organ index and fasting blood glucose values of the T2DM mice,tested the serum levels of triglyceride(TG),total cholesterol(TC),low-density lipoprotein(LDL-C),high-density lipoprotein(HDL-C),malondialdehyde(MDA)and superoxide dismutase(SOD),and performed pathological observations on the liver and pancreas tissues of the mice.Results:All the AMF total flavonoids groups and juice group had significant hypoglycemic effects on STZ-induced T2DM mice(P<0.05),their organ index and TG,TC,LDL-C,HDL-C,MDA,SOD levels(P<0.05)were reduced,and pancreatic and liver tissues were repaired.The AMF total flavonoid group could significantly improve the symptoms called"three polys and one loss"(polyuria,polydipsia,and polyphagia and weight loss)in T2DM mice(P<0.05).The AMF total flavonoids group had the best effect at high dose.Conclusion:AMF has the efficacy of hypoglycemia,hypolipidemia and repair of pancreatic and liver damage in STZ-induced T2DM mice,and the mechanism might be related to its regulation of oxidative stress.
作者
王丽红
王天宇
杨丽
周彤
赵稷
赵宏
张宇
WANG Lihong;WANG Tianyu;YANG Li;ZHOU Tong;ZHAO Ji;ZHAO Hong;ZHANG Yu(College of Pharmacy,Jiamusi University,Jiamusi 154007,China)
出处
《食品工业科技》
CAS
北大核心
2023年第2期386-392,共7页
Science and Technology of Food Industry
基金
佳木斯大学科技创新团队校级创新团队(cxtd202103)
黑龙江省北药与功能食品特色学科建设项目(2018-TSXK-02)。
关键词
黑果腺肋花楸果实
总黄酮
降血糖作用
氧化应激
Ⅱ型糖尿病(T2DM)
血脂代谢
Aronia melanocarpa fruit
total flavonoids
hypoglycemic activity
oxidative stress
type 2 diabetes mellitus(T2DM)
blood lipid metabolism
