摘要
肽(peptide)是指由2~50个氨基酸分子组成,介于小分子和蛋白质之间的物质,具有生物活性多样、易吸收、靶向性强和副作用小等特点,近年来已经成为生物医药研究热点之一。中药中含有大量的肽类物质,传统煎、熬、煮的用药方式,能够有效利用此类成分,发挥其应有的生物活性。而目前实验室中的中药成分研究多数采用高浓度醇提方法,可能造成众多天然药物中肽类成分被忽视。越来越多的研究发现,中药中的肽类成分是发挥传统药效的重要成分。作者结合多年研究以及文献检索,提出了“中药肽——表达中药功效的相对分子质量介于小分子与蛋白之间,由2个或多个氨基酸组成的肽类化合物”的概念,进而总结中药肽的提取与分离、中药肽的结构确定方法与路径,对基于“整体观”和大数据的现代化中药肽研究方法进行了展望,将人工智能预测和高通量筛选技术相结合,提升中药肽发现的效率和准确度,建立不同中药肽和不同靶点之间的全息图像,为基于中医药理论的中药肽创新药物设计及相关健康产品研发提供新范式。
Peptide is a compound consisting of 2-50 amino acids, which is intermediate between small molecule and protein. It is characterized by a variety of biological activities, easy absorption, strong specific targeting, and few side effects and has become one of the hotspots in biomedical research in recent years. Chinese medicine contains a large number of peptides. The traditional processing methods such as decocting and boiling can effectively boost peptides to exert their due biological activities. At present, however, the research on Chinese medicinal components in laboratory generally employs high-concentration alcohol extraction method, which may cause the peptides to be ignored in many natural Chinese medicines. Substantial studies have revealed that the peptides in Chinese medicine are important material basis responsible for the traditional efficacy. Based on years of research and literature retrieval, this study put forward the concept of "traditional Chinese medicine(TCM)-peptides", referring to the components consisting of two or more amino acids with molecular weight between small molecules and proteins that can express the efficacy of Chinese medicine. Furthermore, this study also summarized the extraction and separation of TCM-peptides, and structure determination methods and routes, predicted the research prospect of modern research methods of TCM-peptides based on "holistic view" and big data. The artificial intelligence prediction was combined with high-throughput screening technology to improve the discovery efficiency and accuracy of TCM-peptides, and holographic images between TCM-peptides and biological targets were established to provide references for the innovative drug design and related health product development of TCM-peptides based on TCM theories.
作者
杨晓男
阮丽君
江兴
宋志军
韦坤华
秦双双
梁莹
侯小利
王喜军
缪剑华
YANG Xiao-nan;RUAN Li-jun;JIANG Xing;SONG Zhi-jun;WEI Kun-hua;QIN Shuang-shuang;LIANG Ying;HOU Xiao-li;WANG Xi-jun;MIAO Jian-hua(Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement/Guangxit Engineering Research Center of TCM Resource Intelligent Creation,Guangxi Botanical Garden of Medicinal Plant,Nanning 530023,China;National Engineering laboratory for the Derelopment of Southuestern Endangered Medicinal Materials,Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,China;Engineering Research Center of Efficacy Evaluation and Industrial Derelopment of TCM Classic Formulae of the Ministry of Education,Chinmedomics Research Center of National Administration of Traditional Chinese Medicine,Heilongjiang University of Chinese Medicine,Harbin 150040,China;the Second Afiliated Hospital of Hengyang Medical School,University of South China,Hengyang 421001,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2022年第22期5978-5990,共13页
China Journal of Chinese Materia Medica
基金
广西创新驱动发展专项资金项目(桂科AA18242040)
广西科技基地和人才专项(桂科AD18126013)
中央引导地方科技创新基地建设类项目(桂科ZY21195044)
广西中医药管理局项目(GXZYZ20210016)
广西药用植物园科研创新团队项目(桂药创2019002,桂药创2019006)
广西壮族自治区药用植物园基金项目(桂药园基202001)。
关键词
中药肽
生物活性
研究方法
药物研发
TCM-peptides
bioactivity
research method
drug development
