摘要
四环素类、磺胺类和大环内酯类等抗生素在养猪业应用广泛,主要用于预防和治疗细菌感染。同时,抗生素滥用对环境和人类健康造成的潜在危害逐渐引起人们的关注。为了去除养猪废水中的抗生素,本研究以小球藻和灵芝菌构建了微藻-真菌共生体,分析该共生体对养猪废水中盐酸土霉素(OTC)、盐酸环丙沙星(CPFX)、磺胺二甲嘧啶(SM2)三类抗生素及常规污染物总氮(TN)、总磷(TP)的去除效果,并评价了藻菌共生体的生长性能和光合性能。结果表明,藻菌共生体对OTC有明显的去除效果,去除率高达(89.93±1.70)%,而对SM2和CPFX的去除效果不明显。藻菌共生体对TP、TN、COD的最终去除效率分别是(76.42±1.51)%、(74.41±1.68)%、(46.05±9.30)%。SM2组的藻菌共生体的生长性能最好,第5天叶绿素a浓度达到1 mg/L。OTC组小球藻的光合性能最好,其FV/FM、PIABS、ΨO、ΦEO和ΦDO分别是0.632±0.037、3.103±1.503、0.694±0.041、0.439±0.051和0.368±0.037。藻菌共生体将OTC降解后,微藻的最大光学效率和性能指数开始明显上升。本研究成果将为生物净化技术的实际应用提供理论基础,对于治理水体污染、实现水体生态修复等具有理论指导意义和应用价值。
Tetracyclines,sulfonamides,macrolides and other antibiotics are widely used in the pig industry,mainly for preventing and treating bacterial infections.At the same time,the potential harm caused by the abuse of antibiotics to the environment and human health has gradually attracted people's attention.In order to remove antibiotics from swine wastewater,microalgal-fungal symbionts was constructed with chlorella and ganoderma lucidum in this study.The removal effect of the symbiosis on three kinds of antibiotics,including oxytetracycline hydrochloride(OTC),ciprofloxacin hydrochloride(CPFX)and sulfamethazine(SM2)and conventional pollutants,total nitrogen(TN)and total phosphorus(TP)in swine wastewater was analyzed.The growth performance and photosynthetic performance of microalgal-fungal symbionts were evaluated.The results showed that the microalgal-fungal symbiosis had obvious removal ability on OTC,and the removal rate was as high as(89.93±1.70)%,while the removal effect on SM2 and CPFX was not obvious.The final removal efficiencies of TP,TN and COD by microalgal-fungal symbionts were(76.42±1.51)%,(74.41±1.68)%and(46.05±9.30)%respectively.The growth performance of microalgal-fungal symbionts in SM2 group was the best,and the concentration of chlorophyll a reached 1 mg/L on the fifth day.The photosynthetic performance of Chlorella in OTC group were the best,and FV/FM、PIABS、ΨO、ΦEO andΦDO was 0.632±0.037,3.103±1.503,0.694±0.041,0.439±0.051 and 0.368±0.037,respectively.The maximum optical efficiency and performance index of microalgae began to increase significantly after the OTC was degraded by microalgal-fungal symbiont.The research results will provide a theoretical basis for the practical application of biological purification technology and have theoretical guiding significance and application value for the treatment of water pollution and the realization of water ecological restoration.
作者
徐长江
赵永军
刘娟
刘丽
XU Chang-jiang;ZHAO Yong-jun;LIU Juan;LIU Li(College of pharmacy,Zhejiang University of Technology,Hangzhou,Zhejiang 310014,China;College of Biological,Chemical Sciences and Engineering,Jiaxing University,Jiaxing,Zhejiang 314001,China)
出处
《浙江化工》
CAS
2022年第12期38-46,共9页
Zhejiang Chemical Industry
基金
浙江省基础公益研究计划项目(LQ22C030005)。
关键词
小球藻
藻菌共生体
废水净化
抗生素去除
生长性能
Chlorella
microalgal-fungal symbionts
wastewater purification
removal of antibiotic
growth performance
