摘要
【目的】探究纤维素分解酶对凤梨渣青贮品质及干物质降解率的影响,为凤梨渣的资源化利用提供参考。【方法】选择新鲜凤梨渣,风干至含水量为65%~70%后,将纤维素分解酶按鲜重以0,1500,3000,6000 u/kg添加量添加至凤梨渣中(分为Ⅰ、Ⅱ、Ⅲ、Ⅳ组),混合均匀后装入青贮容器中,每组18个重复,置于通风、避光、干燥处,常温青贮4周后测定常规养分指标和干物质降解率,干物质降解率经体外瘤胃液培养后,通过失重法测定。【结果】添加纤维素分解酶可提高粗蛋白(CP)(P<0.05)、乳酸(LA)(P<0.05)、乙酸(AA)、丙酸(PA)、总挥发性脂肪酸(TVFA)(P<0.05)含量以及乳酸与总挥发性脂肪酸比值(LA/TVFA)(P<0.05)和相对饲用价值(RFV),降低氨态氮(NH3-N)(P<0.05)、粗纤维(CF)、中性洗涤纤维(NDF)(P<0.05)、酸性洗涤纤维(ADF)含量以及氨态氮与总氮比值(NH3-N/TN)(P<0.05)和pH;Ⅱ、Ⅲ、Ⅳ组的CP分别较对照组提高13.86%、19.45%、13.58%;Ⅱ、Ⅲ、Ⅳ组的LA分别较对照组提高21.79%、20.10%、33.83%;Ⅱ、Ⅲ、Ⅳ组的NH3-N和NH3-N/TN分别较对照组降低11.69%、29.87%、24.68%和22.54%、41.97%、33.81%;Ⅱ、Ⅲ、Ⅳ组的RFV较对照组分别提高2.35%、2.85%、3.52%;Ⅱ组亚硝酸盐含量和pH最低。方差分析结果显示,纤维素分解酶添加水平和发酵时间对干物质降解率(DMD)的影响均达到极显著水平(P<0.01);纤维素分解酶添加水平和体外发酵时间与干物质降解率均呈极显著正相关关系(P<0.01);纤维素分解酶添加组干物质降解率均高于对照组;CP、LA、AA、PA、TVFA、LA/TVFA、RFV等指标与DMD呈正相关关系(P<0.05),NH3-N、NH3-N/TN、NDF等指标与DMD呈负相关关系(P<0.05)。【结论】纤维素分解酶可以提高凤梨渣青贮品质和干物质降解率,且以1500~3000 u/kg添加量为宜。
[Objective]This study aims to explore the effect of cellulase on the quality of pineapple residue silage and the degradation rate of dry matter,thus providing a reference for the resource utilization of pineapple residue.[Method]Cellulase were added to pineapple residue with the addition of 0 u/kg,1 500 U/kg,3 000 u/kg and 6 000 u/kg(groups Ⅰ,Ⅱ,Ⅲ and Ⅳ),with 3 repetitions in each group.Samples were taken after silage at room temperature for 4 weeks to determine the fermentation quality and dry matter degradation rate.[Result]The results showed that adding cellulase could increase CP(P<0.05),LA(P<0.05),AA,PA,TVFA(P<0.05),LA/TVFA(P<0.05)and RFV;the adding could decrease NH_(3)-N(P<0.05),NH_(3)-N/TVFA(P<0.05),CF,CF,pH(P<0.05),ADF,NDF,pH;CP and WSC contents in Group Ⅱ,Ⅲ were the highest,NH_(3)-N,NH_(3)-N/TN in Group Ⅲ were the lowest;nitrite contents and pH in Group Ⅱ were the lowest.The results of variance analysis showed that the addition level of cellulase and fermentation time were significantly related to DMD(P<0.01),the addition level of cellulase and Rumen fermentation time were positively correlated with the dry matter degradation rate(P<0.01),and the dry matter degradation rate of the cellulase-added group was higher than that of the Control Group.Based on the results of the experiment and variance analysis,it can be concluded that cellulase can regulate the DMD of pineapple residue silage by increasing CP,LA,AA,PA,TV-FA,LA/TVFA,RFV,and decreasing NH_(3)-N,NH_(3)-N/TN,NDF.[Conclusion]In conclusion,cellulase can improve the silage quality and dry matter degradation rate of pineapple residue,and the appropriate addition amount is 1 500-3 000 u/kg.
作者
王志敬
吴征敏
尹福泉
谭伟军
高振华
赵志辉
WANG Zhijing;WU Zhengmin;YIN Fuquan;TAN Weijun;GAO Zhenhua;ZHAO Zhihui(College of Coastal Agricultural Sciences,Guangdong Ocean University,Zhanjiang,Guangdong 524000,China;Qingyuan Animal Disease Prevention Control Center,Qingyuan,Guangdong 511518,China)
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2022年第6期1399-1406,共8页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家自然科学基金项目(31772562)。
