摘要
枯草芽孢杆菌CY1是一株广谱性的根际促生菌,为促进农业废弃物的资源化利用,提高固态发酵生产生物有机肥过程中枯草芽孢杆菌CY1的扩繁能力,研究采用猪粪堆肥为原料,麸皮、豆粕、玉米粉为辅料进行固态发酵研制新型生物有机肥,以有效活菌数为参考指标,确定原料、辅料最佳配比及接种量。选用Plackett-Burman试验设计对影响发酵的8个因素进行效应评价,筛选出具有显著效应的3个因素:初始pH、发酵温度及含水量。用最陡爬坡试验逼近最大响应区域,通过响应面分析法确定最优发酵工艺参数。结果表明,固态发酵条件为初始pH 7.51、KH_(2)PO_(4)含量0.75%、MgSO_(4)含量0.15%、MnSO_(4)含量0.15%、发酵温度36.10℃、葡萄糖含量6.00%、(NH_(4))_(2)SO_(4)含量0.75%、含水量26.33%,此时固态发酵产物有效活菌数最大,达到4.57×10^(10) CFU·g^(-1)。该研究为利用农业废弃物采用合适的原料配比及工艺条件生产低成本高质量的生物有机肥提供了新途径。
Bacillus subtilis CY1 is a plant broad-spectrum rhizosphere bacterium.To promote the resource utilization of agricultural waste and improve the propagation capacity of Bacillus subtilis CY1 in the production of bio-organic fertilizer by solid fermentation,this study developed a new bio-organic fertilizer by solid fermentation using pig manure compost as raw material and bran,soybean meal and corn meal as auxiliary materials.The optimal ratio of raw materials and excipients and inoculation amount were determined with the number of viable bacteria as reference index.Plackett-burman experiment design was used to evaluate the effects of eight factors on fermentation,and three factors—initial pH,fermentation temperature and water content with significant effects were selected.The steepest climbing test was used to approximate the maximum response area,and response surface analysis was used to determine the optimal fermentation parameters.The results showed that the solid-state fermentation conditions were as follows:initial pH 7.51,KH_(2)PO_(4) content 0.75%,MgSO_(4) content 0.15%,MnSO_(4) content 0.15%,fermentation temperature 36.10℃,glucose content 6.00%,(NH_(4))_(2)SO_(4) content 0.75%,water content 26.33%.At this time,the number of viable bacteria in solid fermentation products reached the maximum,reaching 4.57×10^(10) CFU·g^(-1).This study provides a new way to produce low-cost and high-quality bio-organic fertilizer by using agricultural waste in appropriate raw material ratio and technological conditions.
作者
李彬涛
韦小敏
来航线
李玉龙
王旭东
LI Bintao;WEI Xiaomin;LAI Hangxian;LI Yulong;WANG Xudong(College of Natural Resources and Environment,Northwest A&F University,Yangling,Shannxi 712100,China)
出处
《干旱地区农业研究》
CSCD
北大核心
2023年第1期27-32,44,共7页
Agricultural Research in the Arid Areas
基金
国家重点研发计划资助项目(2018YFD0200405)。
关键词
枯草芽孢杆菌
固态发酵
生物有机肥
响应面分析
Bacillus subtilis
solid state fermentation
bio-organic fertilizer
response surface analysis
