摘要
目的 分析SERPINE1在胃癌组织中的表达及其临床意义。方法 通过检索肿瘤免疫评估资源(TIMER)数据库,了解SERPINE1在33种癌症组织中的表达情况。通过检索癌症基因组图谱(TCGA)数据库,下载375例胃癌组织和32例癌旁组织的RNA-Sep数据和临床信息,通过检索基因综合表达(GEO)数据库GSE54129数据集下载了111例胃癌组织和21例癌旁组织的基因表达谱芯片,分别将TCGA和GSE54129数据集中的SERPINE1进行癌组织和癌旁组织的表达差异分析,COX分析TCGA数据集中临床基线资料和SERPINE1 mRNA表达水平对胃癌患者预后的影响。绘制Kaplan-Meier生存曲线和受试者工作特征曲线(ROC)分析SERPINE1 mRNA表达与预后的关系。通过基因富集分析(GSEA)挖掘并预测SERPINE1参与的通路。结果 TIMER数据库分析表明,SERPINE1在8种癌组织中明显高表达(P<0.05)。TCGA数据集、GSE54129数据集分析表明,在胃癌组织中SERPINE1表达高于癌旁组织(P<0.001)。多因素COX分析表明,病理Stage4、SERPINE1 mRNA表达水平高是影响胃癌患者预后生存时间的独立危险因素[HR(95%CI)=3.061(1.107~8.464)、1.778(1.224~2.582),P<0.05)]。Kaplan-Meier生存曲线分析发现,SERPINE1高表达组总生存期明显低于低表达组(P<0.001)。ROC曲线分析发现,SERPINE1表达预测1、3、5年生存几率的曲线下面积分别为0.598、0.649、0.731。GESA分析说明SERPINE1与上皮间质转化、炎性反应、细胞顶端连接复合物、KRAS通路、血管生成等通路存在相关性。结论 SERPINE1在胃癌组织中表达明显上调,其高表达预示胃癌患者预后不佳,SERPINE1可作为影响胃癌患者预后的一种生物学标志物,并作为潜在的治疗靶点。
Objective To analyze the expression of SERPINE1 in gastric cancer and its clinical significance.Methods The expression of SERPINE1 in 33 kinds of cancer tissues was investigated by searching the TIMER database. By retrieving the TCGA database, the RNA-Sep data and clinical information of 375 gastric cancer tissues and 32 paracancerous tissues were downloaded, and the gene expression profile chips of 111 gastric cancer tissues and 21 paracancerous tissues were downloaded by retrieving the GSE54129 data set of the GEO database. The TCGA data set and SERPINE1 in the GSE54129 data set were respectively used to analyze the expression differences between cancerous tissues and paracancerous tissues, COX analyzed the impact of clinical baseline data and SERPINE1 mRNA expression level on the prognosis of gastric cancer patients in TCGA data set. Kaplan Meier survival curve and ROC were drawn to analyze the relationship between SERPINE1 mRNA expression and prognosis. Gene enrichment analysis(GSEA) was used to explore and predict SERPINE1 involved pathways.Results TIMER database analysis showed that SERPINE1 was significantly highly expressed in 8 kinds of cancer tissues(P<0.05). The analysis of TCGA data set and GSE54129 data set showed that the expression of SERPINE1 in gastric cancer tissues was higher than that in adjacent tissues(P<0.001). Multivariate COX analysis showed that high levels of pathological Stage4 and SERPINE1 mRNA expression were independent risk factors affecting the prognosis and survival time of gastric cancer patients [HR(95% CI)=3.061(1.107-8.464),1.778(1.224-2.582),P<0.05]. Kaplan-Meier survival curve analysis showed that the total survival time of the high expression group of SERPINE1 was significantly lower than that of the low expression group(P<0.001). ROC curve analysis showed that the area under the curve of SERPINE1 expression predicting 1-, 3-, and 5-year survival probability was 0.598, 0.649, and 0.731, respectively. GESA analysis showed that SERPINE1 was correlated with epithelial mesenchymal transformation, inflammatory reaction, cell apical junction complex, KRAS pathway, angiogenesis and other pathways. Conclusion The expression of SERPINE1 in gastric cancer is obviously up-regulated, and its high expression indicates poor prognosis of gastric cancer patients. SERPINE1 can be used as a biological marker affecting the prognosis of gastric cancer patients, and as a potential therapeutic target.
作者
周涛
王笑天
刘东
孔斌
赵士彭
Zhou Tao;Wang Xiaotian;Liu Dong;Kong Bin;Zhao Shipeng(Department of Gastrointestinal Surgery,the Third Hospital of Hebei Medical University,Hebei Province,Shijiazhuang 050091,China)
出处
《疑难病杂志》
CAS
2023年第1期49-53,66,共6页
Chinese Journal of Difficult and Complicated Cases
基金
2019年政府资助临床医学优秀人才培养项目(冀财社[2019]46号)。
