摘要
目的:应用多因素复合建模的方法构建血脂异常痰浊阻遏(PDR)证和脾肾阳虚(SKYD)证小鼠模型并验证该疾病-证候模型的质量。方法:以12只ApoE基因敲除小鼠构建疾病-证候动物模型(PDR组和SKYD组),以6只C57BL/6J小鼠作为正常对照组(NC组)。测定血脂指标,观察宏观行为,对血管内皮巨噬细胞进行转录组学分析。结果:在疾病模型方面,与NC组比较,PDR组和SKYD组TG、TC、LDL-C均显著增高(P<0.05);PDR组和SKYD组间TG、TC、HDL-C、LDL-C指标比较,差异无统计学意义;在证候模型方面,PDR组出现了痰浊阻遏的表现,SKYD组出现了脾肾阳虚的表现;在氧化应激反应方面,PDR组与SKYD组NF-κB信号通路和生物氧化反应差异均有统计学意义(P<0.01)。结论:本研究构建的病证动物模型符合相应病-证的特征性表现,具有较好的病证动物模型质量。
Objective:A multi-factor composite modeling approach was applied to construct mice models of dyslipidemia with phlegm-dampness retention syndrome and spleen and kidney yang deficiency syndrome and to validate the quality of the disease syndrome model.Methods:A total of 12 ApoE^(-/-)mice and establishment of disease-syndrome animal model by multi factor composite modeling method(PDR group and SKYD group).A total of 6 C57BL/6J mice were used as the normal control group(NC group).The indexes of blood lipid were measured,the macro behavior was observed,and the transcriptomic sequencing of vascular endothelial macrophages was analyzed.Results:The quality of disease animal models:Compared with the NC group,TG,TC and LDL-C in the PDR group and the SKYD group were increased(P<0.05).There was no significant statistical difference in TG,TC,HDL-C and LDL-C indexes between the PDR group and the SKYD group.The constructed quality of animal model of syndrome:From the macroscopic behavior,the mice in the PDR group showed the related manifestations of phlegm-dampness retention syndrome,and the mice in the SKYD group showed the related manifestations of spleen and kidney yang deficiency syndrome.The syndrome’s characteristics in animal model of oxidative stress:NF-κB signaling pathway and biological oxidation reaction were the different results which was closely related to oxidative stress(P<0.01).Conclusion:The disease-syndrome animal models constructed in this study conforms to the characteristic performance of corresponding disease and syndrome.The disease-syndrome animal models’quality is good.
作者
陈婧
叶超
王铁山
杨正
李鹏洋
张珊
薛晓琳
CHEN Jing;YE Chao;WANG Tie-shan;YANG Zheng;LI Peng-yang;ZHANG Shan;XUE Xiao-lin(The Third Affiliated Hospital of Beijing University of Chinese Medicine,Beijing 100029,China;Dongzhimen Hospital,Bejing University of Chinese Medicine,Beijing 100700,China;Bejing Institute of Chinese Medicine,Bejing University of Chinese Medicine,Beijing 102488,China;School of Traditional Chinese Medicine,BeijingUniversityof ChineseMedicine,Beijing 102488,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2022年第11期6437-6441,共5页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金青年科学基金项目(No.82004237)
中央高校基本科研业务费专项基金(No.2018-JYBZZ-JS182)。
关键词
血脂异常
脾肾阳虚证
痰浊阻遏证
病证动物模型
模型质量
构建
评价
Dyslipidemia
Spleen and kidney yang deficiency syndrome
Phlegm-dampness retention syndrome
Disease-syndrome animal model
Model quality
Construct
Evaluate
