摘要
目的探讨降真香水提取物对中波紫外线诱导的人角质形成细胞(HaCaT)光损伤的保护作用及机制。方法2018年12月至2020年4月,在广西医科大学基础医学院遗传学实验室进行实验。用前期构建的光老化角质形成细胞模型,用不同浓度降真香水提取物与细胞共培养,用CCK-8法检测细胞增殖率,用试剂盒检测细胞氧化指标活性氧(ROS)、丙二醛(MDA)、超氧化物岐化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、总抗氧化能力(T-AOC)水平,用酶联免疫吸附(ELISA)法检测细胞中白细胞介素(IL)-1β、IL-6、ɑ-肿瘤坏死因子(TNF-α)含量。结果浓度为0.5~2.0 mg/L降真香水提取物对HaCaT细胞增殖有促进作用,与对照组比较,实验组HaCaT细胞增殖率明显升高(P<0.05)。与对照组比较,实验组细胞ROS含量显著降低(F=214.67,P<0.05),MDA含量显著降低(F=811.88,P<0.05),SOD含量升高(F=28.95,P<0.05),CAT含量升高(F=213.31,P<0.05),GPx含量升高(F=65.10,P<0.05),T-AOC水平升高(F=305.58,P<0.05),IL-1β含量降低(F=15.46,P<0.05),IL-6含量降低(F=59.2,P<0.05),TNF-α含量显著降低(F=33.13,P<0.05)。结论0.5~2.0 mg/L降真香水提取物对光老化细胞模型有保护作用,可能与其提高光老化HaCaT细胞内SOD、CAT、GPx等抗氧化酶活力及T-AOC水平,降低ROS、MDA含量,降低炎性细胞因子表达有关。
Objective To investigate the protective effect and mechanism of Acronychia pedunculata water extracts on UV-induced light damage of human keratinocytes.Methods The experiment was conducted from December 2018 to April 2020 in the Guangxi Medical University Laboratory of Genetics.The photoaged keratinocyte model was used,the cells were co-cultured with different concentrations of Acronychia pedunculata water extracts.The cell proliferation rate was detected by CCK-8 method.The levels of reactive oxygen species(ROS),malondialdehyde(MDA),superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GPX)and total antioxidant capacity(T-AOC)of cells were detected by a test kit.The levels of IL-1β,IL-6 and tumor necrosis factor-alpha(TNF-α)were determined by ELISA.Results The proliferation of HaCaT cells was promoted by 0.5 mg/L-2.0 mg/L of the extracts.Compared with control group,the proliferation rate of HaCaT cells in the experimental group was significantly increased(P<0.05).Compared with control group,the contents of ROS was decreased(F=214.67,P<0.05),MDA was decreased(F=811.88,P<0.05),SOD was increased(F=28.95,P<0.05),CAT was increased(F=213.31,P<0.05),GPX was increased(F=65.10,P<0.05),T-AOC was increased(F=305.58,P<0.05),IL-1βwas decreased(F=15.46,P<0.05),IL-6 was decreased(F=59.2,P<0.05),and TNF-αwas decreased(F=33.13,P<0.05).Conclusions The extracts of 0.5-2.0 mg/L of Acronychia pedunculata have protective effects on the photoaging cell model,which may be related to the increase of SOD,CAT,GPX and other antioxidant enzymes and the level of T-AOC in photoaging HaCaT cells,and the decrease of ROS,MDA content and the expression of inflammatory cytokines.
作者
罗秀玲
周源
赵斌斌
黄曼丽
李文宇
范润哥
温斯健
李季超
吴华裕
林有坤
Luo Xiuling;Zhou Yuan;Zhao Binbin;Huang Manli;Li Wenyu;Fan Runge;Wen Sijian;Li Jichao;Wu Huayu;Lin Youkun(Department of Dermatology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530022,China;Department of Dermatology,the People's Hospital of Guangxi Zhuang Autonomous Region,Nanning 530016,China;Department of Dermatology,Guangxi Helanmo Technology Co,LTD,Nanning 530000,China)
出处
《中华医学美学美容杂志》
2022年第6期517-521,共5页
Chinese Journal of Medical Aesthetics and Cosmetology
基金
国家自然科学基金(81760561)
广西自然科学基金(2020GXNSFAA297150)。
关键词
降真香
水提取物
人角质形成细胞
中波紫外线
光老化
Acronychia pedunculatat
Water extract
Human keratinocytes
Ultraviolet B
Photoaging
