摘要
对采自山东省潍坊市滨海新区柽柳防护林的柽柳核纤孔菌(Inocutis tamaricis)进行组织分离和鉴定,利用正交实验确定菌丝生长最适碳源、氮源、pH、温度,进行驯化并测定子实体水提物得率和水提物抗氧化活性。结果表明:在实验范围内,柽柳核纤孔菌菌丝生长最适条件为葡萄糖、酵母膏、pH5.5和35℃;子实体生长发育过程中有明显的后熟阶段(15~20 d),接种85 d左右子实体成熟;子实体水提物得率为2.46%;0.2、0.3mg·mL^(-1)子实体水提物对DPPH自由基清除率较大,0.30 mg·mL^(-1)子实体水提物对ABTS自由基清除率最大。研究结果可为开发和利用柽柳核纤孔菌提供参考。
A wild wood-rotting fungus collected in the coastal Tamarix shelterbelt in Binhai New Urban District, Weifang City, Shandong Province was isolated, identified and then domesticated. Using orthogonal experiments, the optimal carbon source, nitrogen source, pH, and temperature for mycelium growth were determined. Fruiting bodies of artificial cultivation were extracted by water and the resultant water extract was measured for polysaccharide content and antioxidant properties. The results showed that the isolated fungus was Inocutis tamaricis and its optimal carbon source, nitrogen source, pH and temperature for mycelium growth were glucose, yeast extract, pH 5. 5 and 35 ℃, respectively. There was an obvious post-ripening stage(15-20 d), and mature fruiting bodies were formed 85 d after inoculation.The polysaccharide yield of the I. tamaricis water extract was 2.46%. The DPPH scavenging rate of the I. tamaricis water extract was relatively high at 0. 2 mg·mL^(-1) and 0.3 mg·mL^(-1), and the ABTS scavenging rate of the I. tamaricis water extract was the highest at 0.3 mg·mL^(-1). These findings provided a reference for exploring the application of I. tamaricis.
作者
王磊
李世达
崔琪
盖宇鹏
程显好
WANG Lei;LI Shida;CUI Qi;GE Yupeng;CHENG Xianhao(College of Agriculture,Ludong University,Yantai 264001,Shandong,China)
出处
《食用菌学报》
CSCD
北大核心
2023年第1期36-44,共9页
Acta Edulis Fungi
基金
山东省现代农业产业技术体系食用菌产业体系岗位专家项目[鲁农科教字(2021)26号]
东营市第六批黄河三角洲学者项目[东委(2016)84号]。
