摘要
采用组织分离法从贵州安顺发生果斑病的百香果上分离出真菌并纯化,根据柯赫氏法则确定其致病性,明确分离所得菌株B08为致病菌。经形态学观察初步鉴定其为链格孢菌。测定相关基因序列,构建多基因系统发育树,发现B08与梨黑斑链格孢Alternaria gaisen(菌株号:CBS 632.93、LM0519、BMP0243)聚于一支。在室内开展防治药剂的初步筛选,发现氟菌·肟菌酯和苯甲·嘧菌酯对病原菌的抑制效果较好,EC50分别为0.67μg/mL和0.79μg/mL。
The pathogen of passion fruit spot in Anshun of Guizhou was isolated and purified by tissue isolation method.Determined by Koch’s postulates, the isolated strain B08 was identified as the pathogen. It was preliminarily identified as Alternaria by morphological observation. The Alternaria glyceraldehyde-3-phosphate dehydrogenase(gpd), translation elongation factor 1-alpha(TEF1-α), RNA polymerase Ⅱ second largest subunit(RPB2), allergen Alt-a1(Alt-a1), and Calmodulin(Cal) were sequenced, and the phylogenetic tree of multi-gene was constructed. The phylogenetic tree analysis showed that the pathogenic strain B08 and Alternaria gaisen(Strain number: CBS 632.93, LM0519, BMP0243) clustered into one clade. The results of laboratory fungicides screening revealed that the fluopyram·trifloxystrobin and difenoconazole·azoxystrobin had the best inhibitory effects on the pathogen, with EC50values of 0.67 μg/mL and 0.79 μg/mL, respectively.
作者
赵晓珍
王红林
解璞
袁启凤
马玉华
Zhao Xiaozhen;Wang Honglin;Xie Pu;Yuan Qifeng;Ma Yuhua(Institute of Pomology Science,Guizhou Academy of Agricultural Sciences,Guiyang Guizhou 550006,China)
出处
《中国植保导刊》
北大核心
2022年第10期9-14,共6页
China Plant Protection
基金
贵州省农业科学院2018年度学术新苗培养及创新探索专项(黔科合平台人才[2018]5768-14)
贵州省科技计划项目(黔科合服企[2019]4002)。
关键词
百香果果斑病
病原鉴定
链格孢菌
防控药剂
passion fruit spot disease
pathogen identification
Alternaria
fungicides
