摘要
为探究长江中华绒螯蟹雌蟹抱卵前后的食性,在长江口东滩水域采集生殖洄游期中华绒螯蟹雌蟹,采用高通量测序技术,解析其抱卵前后的食物种类组成及其食性特征。试验结果显示,37个食物样品的高质量序列为53 288~90 598个。通过聚类分析共得到423个运算分类单元(OTU),筛选有效序列后,经比对获得的食物类别包括藻类、高等植物、鱼类、甲壳类、软体动物、轮虫、原生动物等。雌蟹抱卵前食物组成中占比较高的类别为高等植物、甲壳动物和鱼类;而在抱卵后食物组成中占比较高的种类为高等植物和藻类。抱卵前的Chao1指数和ACE丰富度指数显著大于抱卵后的,抱卵前、后的Shannon和Simpson多样性指数均无显著性差异。研究结果表明,中华绒螯蟹雌蟹摄食宽度较广,属于机会主义摄食者,亲蟹抱卵前后的食性具有一定的差异性。研究结果揭示了生殖洄游期中华绒螯蟹雌蟹的食物种类组成及雌蟹在抱卵前、后的食性变化特征,并为长江口中华绒螯蟹资源增殖和保护提供科学依据。高通量测序分析技术具有生物检测灵敏度较高的特点,可在一定程度上揭示水生动物的食物种类组成信息,但仍需与传统的胃含物形态学分析方法相结合,以便更准确全面地解析其食性特征。
In order to explore the feeding habits of female Chinese mitten crab Eriocheir sinensis in the Yangtze River before and after spawning, female Chinese mitten crab was collected during reproductive migration in the east beach of the Yangtze River Estuary by single boat single capsule truss bottom trawl, and the food species composition and feeding characteristics were studied before and after spawning by high-throughput sequencing technology. The results showed that the high-quality sequences of 37 samples were varied between 53 288 and 90 598. Through cluster analysis of 423 OTU, the type of live food including algae, higher plants, Piscium, Crustacea, Granulifusus kiranus, rotifer, protozoa were found after screening valid sequences. The main foods were higher plants, Crustacean and Piscium of female crab before spawning, and the main foods were higher plants and algae for female crab after spawning, the abundance index of Chao1 and ACE was significantly higher before spawning than after spawning. The results revealed that the female Chinese mitten crab had a wider feeding width and belonged to opportunistic feeders, with certain differences in the feeding habits of the female Chinese mitten crab before and after spawning. The finding provides a scientific basis for habitat restoration and resource proliferation and protection of Chinese mitten crab in the Yangtze River Estuary. In addition, high-throughput sequencing analysis methods need to be combined with traditional stomach content analysis methods to more accurately and comprehensively analyze food characteristics.
作者
徐静静
冯广朋
张涛
耿智
杨刚
宋超
李春波
XU Jingjing;FENG Guangpeng;ZHANG Tao;GENG Zhi;YANG Gang;SONG Chao;LI Chunbo(Shanghai Engineering Research Center of Fisheries Resources Enhancement and Ecological Restoration of the Yangtze River Estuary,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China)
出处
《水产科学》
CAS
CSCD
北大核心
2023年第1期1-10,共10页
Fisheries Science
基金
国家重点研发计划重点专项(2020YFD0900805)
国家自然科学基金面上项目(31972795)
上海市现代农业产业技术体系项目(沪农科产字2017-4号)。
关键词
高通量测序
中华绒螯蟹
食性
雌蟹
high-throughput sequencing
Eriocheir sinensis
feeding habit
female crab
