摘要
为探究褪黑素(MT)对盐胁迫下黄瓜幼苗生长的影响,以津研四号黄瓜为试材,采用基质盆栽,以150 mmol·L^(-1)NaCl浇灌作为盐胁迫环境,以0 μmol·L^(-1)MT(CK)、50 μmol·L^(-1)MT(T1)、100 μmol·L^(-1)MT(T2)、150 μmol·L^(-1)MT(T3)喷施3叶1心黄瓜为处理组,设置完全空白对照组(CK0)。结果表明,盐胁迫下,黄瓜株高、茎粗、叶面积、抗氧化酶活性、根系活力等均降低,而丙二醛(MDA)含量升高;喷施100 μmol·L^(-1)MT(T2)的黄瓜幼苗盐胁迫缓解效应最佳,与0 μmol·L^(-1)(CK)相比株高增加41.49%(12 d),叶面积增加44.70%(6 d);喷施处理18 d后,与CK相比,T2的茎粗增加了21.24%,叶绿素含量增加了43.85%,根系活力增加了61.03%,过氧化氢酶(CAT)活性提高了3.23倍,MDA含量减少了48.15%。主成分分析显示,100 μmol·L^(-1)MT(T2)较其他浓度对黄瓜幼苗盐胁迫的缓解作用更大,其次是T1和T3。综上,100 μmol·L^(-1)MT(T2)是增强黄瓜幼苗耐盐性的最适浓度。
To explore the effects of melatonin(MT)on the growth of cucumber seedlings under salt stress, this experiment took Jinyan No. 4 cucumber as the test material, adopted matrix pot, irrigated with 150 mmol·L^(-1)NaCl as the salt stress environment. Cucumber was sprayed with 0 μmol·L^(-1)(CK), 50 μmol·L^(-1)(T1), 100 μmol·L^(-1)(T2)and 150 μmol·L^(-1)MT(T3)as treatment groups, and completely blank control group(CK0)was set. The results showed that plant height, stem diameter, leaf area, antioxidant enzyme activity and root activity of cucumber decreased under salt stress, while malondialdehyde(MDA)content increased. Compared with 0 μmol·L^(-1)MT(CK), 100 μmol·L^(-1)MT(T2)had the best salt-stress alleviating effect, and the plant height and leaf area increased by 41.49%(12 d)and 44.70%(6 d)respectively. After 18 days of spray treatment, stem diameter of T2 increased by 21.24%, chlorophyll content increased by 43.85%, root activity increased by 61.03%, catalase(CAT)activity increased by 3.23 times, and MDA content decreased by 48.15% compared with CK. Principal component analysis showed that 100 μmol·L^(-1)MT(T2)had better alleviating effect on salt stress of cucumber seedlings than other concentrations, followed by T1 and T3. In conclusion, 100 μmol·L^(-1)MT(T2)can promote the growth and enhance the salt tolerance of cucumber seedlings.
作者
李荣
焦志阳
银珊珊
周国彦
谢洋
LI Rong;JIAO Zhiyang;YIN Shanshan;ZHOU Guoyan;XIE Yang(Qinhuangdao Vegetable Management Center of Hebei Province,Qinhuangdao 066000,Hebei,China;College of Horticulture Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao 066004,Hebei,China)
出处
《中国瓜菜》
CAS
北大核心
2023年第1期53-58,共6页
China Cucurbits And Vegetables
基金
河北省现代农业产业技术体系露地蔬菜创新团队项目(HBCT2021200210)。
关键词
黄瓜
褪黑素
盐胁迫
生长发育
Cucumber
Melatonin
Salt stress
Growth and development
