摘要
胡萝卜(Daucus carota var. sativa)是一类重要的全球性蔬菜作物。为了全面了解其转录组SSR位点特点,并开发胡萝卜EST-SSR分子标记,本试验对胡萝卜的根、茎、叶混合组织进行转录组测序分析,共获得42 668条Unigene,其中6 611条Unigene序列中含有8 241个SSR位点,SSR发生频率为15.49%,平均分布为0.665 0 kb。主要优势重复序列为二核苷酸,占总SSR的45.35%。以48份胡萝卜为材料,从60对SSR引物中筛选出21对条带清晰稳定、多态性高的引物,其中有11对引物多态信息含量(PIC)大于0.500 0,属于高多态性引物,这些引物占开发引物的52.38%,表明筛选出的21对SSR引物具有较好的遗传多样性。聚类分析可将48份胡萝卜材料在相似系数为0.687 2处分为四类,聚类结果与胡萝卜颜色和产地存在一定的相关性。试验结果为胡萝卜的种质资源鉴定、品种鉴别和分子标记辅助育种等研究提供理论依据。
Carrot(Daucus carota var.sativa)is an important global vegetable crop.In order to fully understand the characteristics of its transcriptome SSR locus,and develop EST-SSR molecular marker for carrot,a total of 42668 Unigenes were obtained based on the transcriptome sequencing data of the mixed tissues(roots,stems,and leaves)of carrot.It was found that 8241 SSR loci were in 6611 of the Unigene sequences,the frequency of SSR was 15.49%,and the average distribution was 0.6650 kb.The type of di-nucleotides SSR was the most abundant,which accounted for 45.35%of the total SSR.The materials include 48 carrot varieties.Twenty-one pairs of primers amplification bands which were clear and good repeatability and high polymorphism were selected from 60 pairs of SSR primers.Among them,11 pairs of primer with the polymorphism information content(PIC)greater than 0.5000 belonged to high polymorphism primers,which accounted for 52.38%of the developed primers,indicating that the selected 21 pairs of SSR primers had a good genetic diversity.UPGMA cluster analysisshowed that 48 carrot varieties could be divided into four groups at the genetic similarity coefficient of 0.687 2,and the clustering results have a certain correlation with carrot color and producing area. The results provided a theoretical basis for identification of germplasm resources, variety identification, and molecular marker assistedbreeding of carrot.
作者
陈春艳
马杰
屈雯
马俊
王磊
杨佼
刘针杉
马维
周禹
孙勃
Chen Chunyan;Ma Jie;Qu Wen;Ma Jun;Wang Lei;Yang Jiao;Liu Zhenshan;Ma Wei;Zhou Yu;Sun Bo(Bijie Institute of Agricultural Science,Bijie,551700;College of Horticulture,Sichuan Agricultural University,Chengdu,611130;Tangshan Food andDrug ComprehensiveTesting Center,Tangshan,063000)
出处
《分子植物育种》
CAS
北大核心
2023年第1期205-214,共10页
Molecular Plant Breeding
基金
贵州省科技厅项目(黔科合支撑[2018]2328号)
贵州省人才办项目(RCJD2020-7)
贵州省科技厅高层次创新型人才项目(毕科人才合字[2021]02号)
知食供应链科技有限公司科技项目(2020008)共同资助
