摘要
目的唾液幽门螺杆菌(Hp)检测分析。方法首先采集样品唾液,将采样品的唾液对其进行预处理,然后放入反应盒内,5min左右后观察其颜色改变,通过比色法进行酶动力学实验,最后进行观察颜色变化来判断是否为阳性。结果本文通过对尿素的浓度以及结合唾液链球菌经过精准的分析后得,经过调节后的液体环境且不会对本实验产生任何影响,所以本实验具有较高的可行度以及较高的可信度,同时对200列试验人员进行精密的检测,然后分析该方法的准确性。结论Hp唾液检测试剂盒灵敏性约为98.29%,特异性约为90.36%,阳性预测值约为94.26%,阴性预测值为约96.15%,准确性约为95%,差异性约5%,低于实验允许变异系数(10%),Hp唾液检测试剂盒实验标本为唾液,同时对人体无危害,且所花费的检测时间短,其操作简单,也可以进行多次检测,适用于家庭、学校以及一些公共场合的初次检查。
Objective To detect and analyze saliva Hp.Methods First,the paper collected sample saliva and pretreated it,put it into reaction box.After about five minutes,color changes were observed.Enzyme kinetics experiment was conducted by colorimetry.Finally,color changes were observed to determine whether it was positive.Results The paper carried out accurate analysis on concentration of urea and streptococcus salivarius.Adjusted liquid environment caused no effect on the experiment,which had high feasibility and reliability.At the same time,the paper carried on precise test for 200 cases test staff,then analyzed accuracy of the method.Conclusion Sensitivity of HP salivary detection kit is about 98.29%,with specificity of about 90.36%,with positive predictive value of about 94.26%,with negative predictive value of about 96.15%,with accuracy of about 95%,with difference of about 5%,lower than allowable coefficient of variation(10%)of experiment.Test sample of HP salivary detection kit is saliva,which is harmLess to human body,with short detection time and simple operation,and it can be tested many times,and is suitable for initial inspection of families,schools and some public places.
作者
李若彬
王柯为
李梦娜
杨贺迪
冷锋
LI Ruobin;WANG Kewei;LI Mengna;YANG Hedi;LENG Feng(Daqing Campus,Harbin Medical University,Daqing,Heilongjiang,163000)
出处
《智慧健康》
2022年第33期6-9,23,共5页
Smart Healthcare
