摘要
从土壤中筛选出抑制哈密瓜细菌性果斑病的拮抗菌株,明确其分类地位,测定其生长条件及防病效果。采用梯度稀释法及平板对峙法进行菌株的分离及筛选,综合形态学观察、生理生化测定以及分子鉴定等多方面对菌株进行鉴定,确定生防菌种属,并测定其最适生长条件,利用平板对峙法测定其抑菌作用,并分别采用针刺接种法、叶面喷施法对生防菌的离体叶片和盆栽防病试验进行测定。本研究从土样中共分离出196株细菌,经对峙筛选发现有20株细菌具有抑菌效果,其中编号为131、791的两株细菌对哈密瓜细菌性果斑病病原菌具有较好的平板拮抗效果,抑菌圈直径分别为(19.03±0.13)mm,(17.55±0.29)mm。经鉴定菌株131为沙福芽孢杆菌(Bacillus safensis),菌株791为贝莱斯芽孢杆菌(B.velezensis)。菌株131的最适生长条件为NB培养基,培养18 h,温度37℃,pH 7,接种量为2%。菌株791的最适生长条件为KB培养基,培养18 h,温度37℃,pH 6,接种量为1%。菌株131和791对哈密瓜细菌性果斑病的离体叶片防效分别为83.33%和87.53%,室内盆栽防效分别为69.86%和77.99%,同时也对多种病原真菌具有抑制作用。菌株131和791均为对哈密瓜细菌性果斑病具有较好防效的芽孢杆菌(Bacillus),对瓜类作物细菌性果斑病的防治提供了理论补充,奠定了果斑病害生物防治的基础。
The antagonistic strains against bacterial fruit blotch(BFB)of cantaloupe were screened from soil,their taxonomic status was clarified,and their growth conditions and disease prevention effects were determined.Separation and screening were carried out by plate dilution method and confrontation method,and the species was identified by comprehensive morphological,physiological,biochemical and molecular identification.After determining the species of biocontrol bacteria,the optimal growth conditions were determined,and the bacteriostatic effect was determined by the plate confrontation method.The leaves and potted plants of biocontrol bacteria were measured by acupuncture inoculation method and foliar spraying method respectively.In this study,196 strains of bacteria were isolated from soil samples,20 strains of them were found to have bacteriostatic effect via confrontation method.Among them,two strains numbered 131 and 791 had better plate antagonism effect on the pathogen of BFB,and the diameters of the inhibition zones were(19.03±0.13)mm and(17.55±0.29)mm,respectively.The strain 131 was identified as Bacillus safensis and strain 791 as B.velezensis.The optimal growth condition of strain 131 was NB medium,cultured for 18 h,temperature was 37℃,pH 7 and the inoculum was 2%.The optimal growth condition of strain 791 was KB medium,cultured for 18 h,temperature was 37℃,pH 6 and the inoculation amount was 1%.Strain 131 and 791 were 83.33%and 87.53%in vitro,69.86%and 77.99%in indoor pots,respectively;meanwhile they had inhibitory effects on various pathogens.In summary,strain 131 and 791 are Bacillus with good control effect on BFB,which provides theoretical supplements for the prevention and control of BFB and lays a solid foundation for BFB.
作者
张玲
张荣意
刘盛科
谭志琼
ZHANG Ling;ZHANG Rong-yi;LIU Sheng-ke;TAN Zhi-qiong(School of Life Sciences,Hainan University,Haikou 570228)
出处
《生物技术通报》
CAS
CSCD
北大核心
2023年第1期253-263,共11页
Biotechnology Bulletin
基金
海南省自然科学基金项目(318MS011)。
关键词
哈密瓜细菌性果斑病
拮抗
鉴定
盆栽防效
抑菌作用
最适生长条件
bacterial fruit blotch of cantaloupe
antagonism
identification
control effect on potted plant
bacteriostatic effect
optimal growth conditions
