摘要
为了开发樱属植物的基因组SSR(简单重复序列)分子标记,本研究在IlluminaNovaSeq PE平台上进行喜马拉雅樱花(Cerasus cerasoides)基因组序列勘测。利用Perl脚本MISA识别微卫星序列,并随机合成100对引物进行引物多态性筛选。选择易扩增、多态性高的引物,采用荧光标记毛细管电泳法在45种樱属植物中进行检测和遗传多样性分析。结果表明,喜马拉雅樱花基因组共存在113 571个SSR位点(≥5个重复),其中包含53.31%单核苷酸基序,36.64%二核苷酸基序,其余基序类型占比10%。在此基础上共筛选出21个SSR标记,并将其用于观赏樱属植物的遗传多态性评估,结果共检测到215个等位基因,平均每个位点10.24个等位基因,平均杂合度为0.38。采用SSR标记构建的进化树较好地反映了45种供试樱属植物之间的亲缘关系。本研究开发了全新的樱花基因组SSR分子标记,可用于樱属植物的遗传多样性分析和分子标记辅助育种。
In order to develop genome-SSR(Simple sequence repeat) molecular markers of Cerasus, the genome sequence survey of Cerasus cerasoides was done on IlluminaNovaSeq PE in this study. Microsatellite sequences were identified by using Perl script MISA, and 100 pairs of primers were synthesized randomly for primer polymorphism screening. Then the primers with easy amplification and high polymorphism were selected to detect and analyze the genetic diversity of 45 flowering cherries by fluorescence labeled capillary electrophoresis. The results showed that there were 113 571 SSR loci(≥5 repeats) including 53.31% single nucleotide motifs, 36.64% dinucleotide motifs and 10% other motifs. 21 SSRs were developed and their polymorphisms were evaluated in flowering cherries. A total of 215 alleles were detected. The mean number of alleles per locus was 10.24 and the mean heterozygosity was 0.38. The phylogenetic tree constructed by SSR markers could well reflect the genetic relationship among 45 tested Cerasus species. In this study, new SSR molecular markers were developed, which can be used for genetic diversity analysis and molecular marker assisted breeding of Cerasus.
作者
李水根
关媛
李秀芬
朱继军
李记开
朱建军
Li Shuigen;Guan Yuan;Li Xiufen;Zhu Jijun;Li Jikai;Zhu Jianjun(Forest&Fruit Tree Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai,201403;Crop Breeding&Cultivation Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai,201403;Shanghai Botanical Garden,Shanghai,200231)
出处
《分子植物育种》
CAS
北大核心
2022年第24期8160-8169,共10页
Molecular Plant Breeding
基金
上海市种业发展项目(沪农科种字[2017]第1-6号)
上海市市级农口系统青年人才成长计划项目(沪农青字[2018]第1-9号)共同资助。
