摘要
建立了一种利用高效液相色谱同时测定化妆品中甲酚曲唑(UV-P)、奥克三唑(UV-329)、布美三唑(UV-326)、甲酚曲唑三硅氧烷(DTTSO)、亚甲基双-苯并三唑基四甲基丁基酚(UV-360)的方法。其中,水状化妆品经甲醇/四氢呋喃溶解、超声提取、微孔滤膜过滤后进样;乳液状、霜状化妆品经甲醇/四氢呋喃溶解、涡旋振荡、超声提取、微孔滤膜过滤后进样。使用ZORBAX SC-C18色谱柱(250 mm×4.6 mm,5μm),柱温23℃,以0.1%的甲酸水溶液为A相、甲醇为B相、四氢呋喃为C相进行洗脱,流速1.0 mL/min,检测波长340 nm,进样量5μL。结果表明,5种紫外线吸收剂在11~176 mg/L的质量浓度范围内线性关系良好(R2≧0.9994),检测限分别为6.72,11.94,13.80,18.47和23.49μg/L,相对标准偏差小于1.7%(n=6),加标平均回收率达到93.7%~104.5%。该方法能满足化妆品中5种苯并三唑类紫外线吸收剂的测定。
A method for the determination of 2-(2-benzotriazolyl)-4-methylphenol(UV-P),2-benzotriazol-2-yl-4-(1,1,3,3-tetramethylbutyl)phenol(UV-329),2-tert-butyl-6-(5-chloro-2Hbenzotriazol-2-yl)-4-methylphenol(UV-326),2-(benzotriazol-2-yl)-4-methyl-6-[2-methyl-3-[methyl-bis(trimethylsilyloxy)silyl]propyl]phenol(DTTSO)and 2,2-methylenebis[6-(2H-benzotriazol-2-yl)-4-(1,1,3,3-tetramethylbutyl)phenol](UV-360)in cosmetics by high performance liquid chromatography was established.Watery cosmetics were dissolved in methanol/tetrahydrofuran,ultrasonically extracted,and filtered by microporous membrane for injection.Emulsion and cream cosmetics were prepared by methanol/tetrahydrofuran dissolution,vortex oscillation,ultrasonic extraction,and microporous membrane filtration injection.The target chemicals were separated on ZORBAX SC-C18 column(250 mm×4.6 mm,5μm)using 0.1%formic acid,methanol and tetrahydrofuran as mobile phases and detected at 340 nm and 23℃.The flow rate of the mobile phase was 1.0 mL/min and the injection volume was 5μL.The results show that the five benzotriazole ultraviolet absorbents have a good linear relationship in the mass concentration range of about 11-176 mg/L(R2≧0.9994),the detection limits are 6.72,11.94,13.80,18.47 and 23.49μg/L,respectively,the relative standard deviation is less than 1.7%(n=6),and the average recovery rate is 93.7%-104.5%.The method is suitable for the determination of five benzotriazole ultraviolet absorbents in cosmetics.
作者
王磊
吴越
田冬
Lei Wang;Yue Wu;Dong Tian(Department of Food and Environmental Engineering,Chuzhou Polytechnic,Chuzhou,Anhui 239000,China;School of Chemistry and Materials Engineering,Huainan Normal University,Huainan,Anhui 232000,China)
出处
《日用化学工业（中英文）》
CAS
北大核心
2023年第1期109-114,共6页
China Surfactant Detergent & Cosmetics
基金
安徽省高校自然科学研究重点项目(KJ2021A1406)
安徽省高校优秀青年骨干教师国内访问研修项目(gxgnfx2021201)
滁州职业技术学院校级重点科研项目(YJZ-2020-03)。
关键词
超声提取
高效液相色谱
化妆品
苯并三唑
防晒剂
紫外线吸收剂
ultrasonic extraction
high performance liquid chromatography
cosmetic
benzotriazole
sunscreen
ultraviolet absorbent
