摘要
[目的]开展3年生滇牡丹(Paeonia delavayi)实生苗的分级研究,为人工规模化壮苗培育和确定合理的滇牡丹种苗质量分级标准提供科学依据。[方法]对滇牡丹5个地理种源3年生的实生苗进行随机取样,测量苗木的地径、株高、主根长和一级侧根数等7个形态指标,采用平均值±1/2标准差法、逐步聚类法和K-均值聚类法对其进行分级研究。[结果]主成分分析显示,滇牡丹实生苗的地径(D)和一级侧根数(B)2项指标的主成分贡献率分别为63.48%和11.63%,累计贡献率达75.11%,因此D和B可作为3年生滇牡丹种苗等级划分的主要指标,不同等级的苗木存在显著差异。3种不同聚类方法中,各级苗木分级范围无显著区别,3年生滇牡丹实生苗分级指标为:Ⅰ级苗:D≥7.24 mm,B≥6;Ⅱ级苗:4.16mm≤D<7.24mm,3≤B<6;Ⅲ级苗:D<4.16mm,B<3。[结论]不同地理种源间的实生苗生长性状具有显著区别,DNL种源株高生长发育最快;DYR种源地径生长最快;DYR种源苗期质量等级最好,Ⅰ级和Ⅱ级苗出圃率最好,可达70%;DDC种源苗期质量最差,Ⅰ级和Ⅱ级苗出圃率仅为35.95%。
[Objective]Carry out 3 years of quality evaluation of Paeonia delavayi seedlings to provide technical support for the cultivation of strong seedlings and artificial scale development.Principal component analysis method was used to determine the seedling grading index of Paeonia delavayi.[Method]The seedlings of 5 geographical provenances of peony in Yunnan were randomly sampled for 3 years,and 7 morphological indicators such as ground diameter,plant height,root length and primary lateral roots of the seedlings were measured,and the ratio and standard of each grade of seedlings were compared and analyzed by means of±1/2 standard deviation method,stepwise clustering method and K-mean clustering method to determine the reasonable quality of peony seedlings classification methods and classification standards.[Result]The contribution rates of the principal components of the ground diameter(D)and the number of first-level lateral roots(B)2 traits were 63.48% and 11.63%,respectively,and the cumulative variance contribution rate was 75.11%.D and B could be used as the main indexes for grading 3-year-old Paeonia delavayi.There were significant differences among seedlings of various grades.There was no significant difference in the proportion of seedlings of each grade and the range of grades of Paeonia delavayi determined by three different methods.Based on the seedling ratio of each grade,the range of grade values and the actual seedling production process,the quality grading of 3-year-old Paeonia delavayi seedlings should adopt the average±1/2 standard deviation method.The grading standard was:Grade Ⅰ seedlings,D≥7.24 mm,B≥6;Grade Ⅱ seedling:4.16 mm≤D<7.24 mm,3≤B<6;Grade Ⅲ seedling:D<4.16 mm,B<3.[Conclusion]There were obvious differences in the trait performance between different provenances.The DNL provenance grew the fastest in plant height,and the DYR provenance grew the fastest in ground diameter;DYR provenance had the best seedling quality grade,Ⅰ and Ⅱ seedlings had the best rate of nursery emergence,up to 70%;DDC provenance had the worst seedling quality of the leaving nursery rate Ⅰ and Ⅱ seedlings was only 35.95%.
作者
普甜
宋静
张志华
陈婉东
王娟
PU Tian;SONG Jing;ZHANG Zhi-hua(Forestry College,Southwest Forestry University,Kunming,Yunnan 650224;College of Landscape and Horticulture,Southwest Forestry University,Kunming,Yunnan 650224)
出处
《安徽农业科学》
CAS
2022年第24期98-102,共5页
Journal of Anhui Agricultural Sciences
基金
云南省重大基础专项“生物资源数字化开发应用”(202002-AA10007)
云南省万人计划“云岭产业技术领军人才”专项(云发改[2018]212号)
云南省科技计划-国际合作项目(2015IA005)。
关键词
滇牡丹
地理种源
平均值±1/2标准差法
逐步聚类法
K-均值聚类法
生长差异
Paeonia delavayi
Geographic provenance
Mean±1/2 standard deviation method
Stepwise clustering method
K-means clustering method
Growth difference
