摘要
溃疡性结肠炎(ulcerative colitis,UC)是一种特发性结肠炎症,会引起腹泻、便血、腹痛等症状,其发病率不断上升。现有研究表明氧化应激是造成UC的重要原因之一,而超氧化物歧化酶(superoxide dismutase,SOD)在缓解UC症状上有一定的效果。因此,本实验通过加热SOD使其自组装形成SOD纳米颗粒(记作ΔSOD),以提高SOD的生物利用度,通过理化指标分析发现ΔSOD纳米颗粒的活力、细胞跨膜能力以及自由基清除能力较天然SOD均显著提高;再采用逆向蒸发法制备ΔSOD脂质体(记作L-ΔSOD),并灌胃UC模型小鼠,结果显示,与阳性对照组和模型组相比,L-ΔSOD可以显著改善UC,降低小鼠疾病活动指数评分,恢复小鼠结肠组织状态,增加结肠长度,降低结肠密度和脾脏系数,恢复黏膜上皮完整性;同时L-ΔSOD可以降低结肠组织MDA含量,并通过减少细胞促炎因子质量浓度、提高谷胱甘肽过氧化物酶活力和紧密连接蛋白mRNA水平来改善UC。综上,L-ΔSOD对UC具有较好的改善作用,本实验可为SOD促进人体健康的实际应用提供参考。
With an increasing incidence rate,ulcerative colitis(UC)is an idiopathic inflammation of the colon causing diarrhea and blood in the stool.Existing studies have shown that oxidative stress is an important factor leading to UC,while superoxide dismutase(SOD)has a certain effect on relieving UC symptoms.To improve its bioavailability,superoxide dismutase(SOD)was self-assembled into nanoparticles(ΔSOD)by heating it.The activity,transmembrane capacity and radical scavenging capacity ofΔSOD were significantly better than those of native SOD.ΔSOD liposomes(L-ΔSOD)were prepared by reverse-phase evaporation and orally administered to mice with UC.The results showed that compared with the positive control and model groups,L-ΔSOD significantly ameliorated UC,reduced disease activity index(DAI)score,restored the morphology of colon tissue,increased the length of colon,reduced the density of colon and spleen coefficient,and restored the integrity of mucosal epithelium.Besides,L-ΔSOD could reduce the contents of malondialdehyde(MDA)and pro-inflammatory factors in colon tissue and increase the levels of glutathione peroxidase(GSH-Px)and tight junction protein mRNA expression.These results confirmed that oral L-ΔSOD has a good ameliorative effect on UC,which will lay the foundation for the application of SOD in promoting human health.
作者
黎俏灵
李鹤年
郭静科
朱则亮
滕薇
胡雨嘉
罗圆圆
王梦田
刘树滔
LI Qiaoling;LI Henian;GUO Jingke;ZHU Zeliang;TENG Wei;HU Yujia;LUO Yuanyuan;WANG Mengtian;LIU Shutao(Institute of Biotechnology,Fuzhou University,Fuzhou 350002,China;Zhicheng College,Fuzhou University,Fuzhou 350002,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2023年第1期166-174,共9页
Food Science
基金
福建省科技厅产业技术开发与应用计划:区域发展项目(2018N3009)
“十三五”国家重点研发计划重点专项(2016YFD0400202)。
