摘要
WRKY转录因子是植物逆境研究中最广泛的家族之一,在植物响应生物胁迫和非生物胁迫中具有重要作用。本研究利用本地BLAST对牛樟基因组进行比对分析得到58个牛樟WRKY基因序列,并对牛樟WRKY转录因子进行理化性质、保守基序、系统进化及表达谱分析。保守结构域和基序预测发现,牛樟转录因子家族蛋白主要分为三大类,即Ⅰ、Ⅱ、Ⅲ类,多数蛋白较保守,其中Ⅰ、Ⅱ类中有两个蛋白(CkWRKY25,CkWRKY1)存在Q残基的变异;CkWRKY基因表达谱数据分析发现,有22个基因在受干旱、低温胁迫时上调表达,占牛樟WRKY家族的37.9%,其中18个基因受干旱胁迫呈高表达水平,占上调基因的81.8%,4个基因在4℃低温胁迫下表达量显著升高,占比18.2%。将牛樟中22个表达差异显著的基因与14个具有抗旱、抗寒WRKY基因共同建树,结果显示36个WRKYs蛋白聚为两大类,参与干旱及低温胁迫的蛋白主要集中在第一大类中,其中CkWRKY26、CkWRKY38、CkWRKY39、CkWRKY45、CkWRKY55分别和参与干旱胁迫的蛋白聚为一支,且C端锌指结构均为C2H2;CkWRKY20、CkWRKY54和参与低温胁迫中陆地棉的GhWRKY62、毛果杨的PtWRKY34、西洋参的PqWRKY7、旱地棉的GaWRKY28、陆地棉的GhWRKY73以及小麦的TaWRKY19和TaWRKY10聚为一支。本研究基于牛樟高通量转录组数据,探究牛樟WRKY转录因子家族生物学特性,为深入研究牛樟WRKY基因非生物胁迫调控提供科学依据。
WRKY transcription f ac tor is one of the most extensive family of plant stress studies and plays an important role in plant response to biotic and abiotic stresses.In this study,58 WRKY gene sequences of Cinnamomum kanehirai were obtained by comparing and analyzing the genome of C.kanehirai using local BLAST,and the physical and chemical properties,conserved motif,phylogenetic analysis and expression profile of WRKY transcription factors of C.kanehirai were analyzed.The results of conservative domains and motif analysis find that C.kanehirai transcription factor family proteins can be divided into three categories including classⅠ,Ⅱ,Ⅲ;most proteins are conservative,Ⅰ,Ⅱclass has two proteins(CkWRKY25,CkWRKY1)Q residues.Analysis of the CkWRKY gene expression profile data showed that 22 genes were up-regulated under drought and low temperature stress,accounting for 37.9%of the WRKY family.Among them,18 genes were highly expressed under drought stress,accounting for 81.8%of the up-regulated genes,and 4 genes were significantly increased under low temperature stress at 4℃,accounting for 18.2%.22 genes with significantly different expression levels and 14drought-resistant and cold-resistant WRKY genes in C.kanehirai jointly built evolutionary trees.The results showed that the 36 WRKYs proteins were clustered into two groups,and the genes involved in drought and low temperature stress were mainly concentrated in the first group.Among them,CkWRKY36,CkWRKY38,CkWRKY39,CkWRKY45,CkWRKY55 and the proteins involved in drought stress were clustered into one branch respectively,and the structure of C-terminal zinc finger was C2H2.CkWRKY20,CkWRKY54 and GhWRKY62,GhWRKY73of Gossypium hirsutum,PtWRKY34 of Populus trichocarpa,PqWRKY7 of Panaxquin qufolius,GaWRKY28 of Gossypium aridum,TaWRKY19 and TaWRKY10 of Triticum aestivum of were all gathered together.This study was based on high-throughput transcriptome data from C.kanehirai,the biological characteristics of C.kanehirai WRKY transcription factor family were explored to provide scientific basis for in-depth study of abiotic stress regulation of C.kanehirai WRKY gene.
作者
张璋
王毅
罗玛妮娅
郑元
Zhang Zhang;Wang Yi;Luo Maniya;Zheng Yuan(College of Forestry,Southwest Forestry University,Kunming,650224;The Key Laboratory of Rare and Endangered Forest Plants of State Forestry Administration,Laboratory of Forest Plant Cultivation and Utilization,Yunnan Academy of Forestry and Grassland,Kunming,650201)
出处
《分子植物育种》
CAS
北大核心
2022年第21期7025-7035,共11页
Molecular Plant Breeding
基金
云南省重大科技专项计划食用菌项目子课题(202002AE320003)
云南省基础研究计划面上项目(2021FB213
2021FB045)
云南省博士后科研基金项目(188102)
国家自然科学基金项目(31860177)
分子生物学研究团队(LKYTD-2020-001)共同资助。
关键词
牛樟
WRKY转录因子
转录组分析
干旱与低温胁迫
Cinnamomum kanehirai
WRKY transcription factors
Transcriptome analysis
Drought and low temperature stress
