摘要
目的观察慢性束缚应激肝郁脾虚证大鼠血清白细胞介素-6(IL-6)含量及下丘脑、胃、结肠组织Ⅳ型胶原蛋白A2(COL4A2)、Ⅸ型胶原α1链(COL9A1)m RNA表达的变化及逍遥散的调节作用。方法将50只雄性SD大鼠随机分为正常组、7d和21d模型组、7d和21d逍遥散组,每组10只,以慢性束缚应激方法制作肝郁脾虚证大鼠模型并用逍遥散进行干预。观察各组大鼠宏观表现、摄食量及体重增长情况。造模7d和21d结束后,采用间苯三酚法测定各组大鼠血清D-木糖含量,ELISA法检测血清IL-6含量,实时荧光定量PCR检测下丘脑、胃、结肠组织COL4A2、COL9A1的m RNA表达。结果与正常组比较:7d和21d模型组大鼠摄食量和体重增长量显著减少(P<0.01),血清IL-6含量显著升高(P<0.01),下丘脑、胃组织COL4A2m RNA表达显著升高(P<0.01),结肠组织COL4A2 m RNA表达显著降低(P<0.05或P<0.01),下丘脑、胃、结肠组织COL9A1mRNA表达均显著降低(P<0.05或P<0.01);21d模型组大鼠血清D-木糖含量显著降低(P<0.01)。逍遥散对模型组大鼠的上述变化均具有一定的调节作用(P<0.05或P<0.01)。结论调节炎症因子IL-6及胶原蛋白COL4A2、COL9A1的异常表达可能是逍遥散防治慢性应激肝郁脾虚证大鼠的机制之一。
Objective To observe the content of interleukin-6(IL-6)in serum and collagen-typeⅣA2(COL4A2)and collagen-typeⅨα1 chain(COL9A1)in hypothalamus,stomach and colon of rats with liver stagnation and spleen deficiency syndrome under chronic binding stress.Methods Fifty male SD rats were randomly divided into normal group,7d and 21d model group,7d and 21d Xiaoyaosan group,10 rats in each group.Rat models of liver depression and spleen deficiency were made by chronic restraint stress method and Xiaoyao Powder was used to intervene.Observe the macroscopic performance,food intake and weight gain of rats in each group.After 7 days and 21 days of modeling,the phloroglucinol method was used to determine the serum D-xylose content,the ELISA method was used to detect the serum IL-6 content,and the real-time fluorescence quantitative PCR was used to detect the hypothalamus,stomach,and colon tissues COL4A2,COL9A1 MRNA expression.Results Compared with normal group,the food intake and weight gain of the rats in 7d and 21 model group were significantly reduced(P<0.01),the serum IL-6 content was significantly increased(P<0.01),and the expression of COL4A2mRNA in hypothalamus and gastric tissue was significantly increased(P<0.01),the expression of COL4A2mRNA in colon tissue was significantly decreased(P<0.05 or P<0.01),and the expression of COL9A1mRNA in hypothalamus,stomach,and colon tissues were significantly decreased(P<0.05 or P<0.01);the content of serum D-xylose in 21d model group decreased significantly(P<0.01).Xiaoyao Powder has a certain regulatory effect on the above changes in rats of model group(P<0.05 or P<0.01).Conclusion Regulating the abnormal expression of inflammatory factor IL-6 and collagen COL4A2 and COL9A1 may be one of the mechanisms of Xiaoyao Powder preventing and treating chronic stress rats with liver depression and spleen deficiency.
作者
杨力强
蒋春建
龙春莉
肖华业
连利军
李熠毅
李晓红
YANG Li-qiang;JIANG Chun-jian;LONG Chun-li;XIAO Hua-ye;LIAN Li-Jun;LI Yi-yi;LI Xiao-hong(Guangxi University of Chinese Medicine,Nanning 530200;Guangxi Key Laboratory of ChineseMedicine Foundation Research,Guangxi University of Chinese Medicine,Nanning 530200)
出处
《湖北中医药大学学报》
2022年第6期13-17,共5页
Journal of Hubei University of Chinese Medicine
基金
国家自然科学基金项目(项目编号:81560750、81860817)
广西自然科学基金(项目编号:2018GXNSFAA138018)
广西中医基础研究重点实验室自主研究课题(项目编号:20-065-53-06)
中医学广西一流学科桂教科研[2018]12号(项目编号:2018XK017)。
