摘要
目的探讨药物性肝损伤(DILI)患者血清微小RNA(miR)-21和miR-124a水平变化及其临床意义.方法2019年3月~2022年3月我院收治的87例DILI患者和60例同期体检的健康人,采用实时荧光定量PCR法检测血清miR21和miR-124a mRNA水平,采用ELISA法检测核转录因子-κB(NF-κB)和白介素-6(IL-6)水平.结果在DILI发生时,血清ALT、AST、GGT和TBIL峰值水平分别为(143.6±51.8)U/L、(158.2±38.8)U/L、(131.6±26.8)U/L和(41.9±9.6)μmol/L;DILI患者血清miR-21、miR124a mRNA、NF-κB和IL-6水平分别为(1.4±0.3)、(2.6±0.4)、(3615.4±526.4)pg/ml和(12.7±1.8)pg/ml,均显著高于健康人[分别为(1.0±0.1)、(1.1±0.1)、(692.2±144.6)pg/ml和(3.4±0.7)pg/ml,P<0.05];混合型DILI患者血清NF-κB和IL-6水平分别为(3874.5±282.5)pg/ml和(14.4±2.6)pg/ml,均显著高于肝细胞型DILI患者[分别为(3609.8±296.4)pg/ml和(12.6±1.5)pg/ml,P<0.05]或胆汁淤积型DILI患者[分别为(3437.2±253.7)pg/ml和(11.7±1.3)pg/ml,P<0.05];混合型、肝细胞型和胆汁淤积型DILI患者血清miR-21和miR-124a水平无显著性差异(P>0.05).结论DILI患者血清miR21和miR-124a水平随肝功能损伤的出现而升高,但在三型不同肝损伤类型患者其水平无显著性差异,对临床分型的判断无指导意义.
Objective The aim of this study was to explore the changes of serum microRNA(miR)-21 and miR-124a levels in patients with drug-induced liver injury(DILI)and their clinical implications.Methods 87 patients with DILI and 60 health persons proven by physical examination were enrolled in our hospital between March 2019 and March 2022.Serum miR-21 and miR-124a levels were detected by real-time fluorescence quantitative PCR,and serum nuclear transcription factor-κB(NF-κB)and interleukin-6(IL-6)levels were determined by ELISA.Results Serum peak ALT,AST,GGT and bilirubin levels in patients with DILI in our series were(143.6±51.8)U/L,(158.2±38.8)U/L,(131.6±26.8)U/L and(41.9±9.6)μmol/L;serum miR-21,miR124a mRNA,NF-κB and IL-6 levels in patients with DILI were(1.4±0.3),(2.6±0.4),(3615.4±526.4)pg/ml and(12.7±1.8)pg/ml,all significantly higher than[(1.0±0.1),(1.1±0.1),(692.2±144.6)pg/ml and(3.4±0.7)pg/ml,respectively,P<0.05]in healthy persons;serum NF-κB and IL-6 levels in patients with mixed DILI were(3874.5±282.5)pg/ml and(14.4±2.6)pg/ml,both much higher than[(3609.8±296.4)pg/ml and(12.6±1.5)pg/ml,respectively,P<0.05]in patients with hepatocyte injury or[(3437.2±253.7)pg/ml and(11.7±1.3)pg/ml,respectively,P<0.05]in DILI patients with cholestasis;unfortunately,there were no significant differences as respect to serum miR-21 and miR-124a levels among patients with different clinical types(P>0.05).Conclusion Serum miR-21 and miR-124a levels in patients with DILI significantly increase as liver function test deteriorated,but their clinical implications is still under investigation.
作者
林仕文
韩锋
魏玉
陈文传
Lin Shiwen;Han Feng;Wei Yu(Clinical Laboratory,People's Hospital,Wanning 571500,Hainan Province,China)
出处
《实用肝脏病杂志》
CAS
2023年第1期43-46,共4页
Journal of Practical Hepatology
基金
海南省自然科学基金资助项目(编号:821MS0779)
