摘要
【目的】研究TIFY基因家族在月季(Rosa chinensis Jacq.)干旱胁迫与盐胁迫发生过程中的作用。【方法】利用生物信息学方法对月季TIFY家族成员进行全基因组鉴定、系统进化树分析、理化性质分析、染色体定位分析、基因共线性分析和基因结构分析,并结合转录组表达数据以及顺式元件分析筛选与盐胁迫、干旱胁迫响应相关的基因。【结果】鉴定了13个月季TIFY家族成员;通过与拟南芥、水稻共同构建的进化树,将这些成员分为4个亚家族;这些成员分布在5条染色体上,与拟南芥同源性更高;各亚家族特征结构域完整。其中6个RcTIFY成员在干旱胁迫和盐胁迫中发挥着积极作用;顺式元件结果表明,大多数RcTIFY成员可能参与了脱落酸与茉莉酸甲酯的响应。积极响应干旱胁迫或盐胁迫的TIFY家族成员RcTIFY5a、RcTIFY5b、RcTIFY6a、RcTIFY6b、RcTIFY10b均含有丰富的脱落酸、茉莉酸甲酯响应元件。【结论】月季中有13个RcTIFY基因,多个成员在干旱胁迫和盐胁迫响应中发挥着积极作用,脱落酸和茉莉酸甲酯是该家族成员的潜在调控激素。
【Objective】To investigate the role of TIFY gene family in the occurrence of drought stress and salt stress in Rose(Rosa chinensis Jacq.). 【Method】The genome-wide identification, phylogenetic tree analysis, physicochemical property analysis, chromosomal localization analysis, gene collinearity analysis, and gene structure analysis of TIFY family members were performed using bioinformatics methods, and transcriptome expression data and cis-element analysis were combined to screen genes associated with salt stress and drought stress response. 【Result】In this study, a total of 13 members of the Rose TIFY family were identified;These members were divided into four subfamilies through an evolutionary tree constructed jointly with Arabidopsis and Oryza;these members were distributed on five chromosomes with higher homology to Arabidopsis;the characteristic structural domains of each subfamily were complete. Six of the RcTIFY members play active roles in drought stress and salt stress;the cis-element results indicated that most of the RcTIFY members may have been involved in the response to abscisic acid and methyl jasmonate. The TIFY family members RcTIFY5a, RcTIFY5b, RcTIFY6a, RcTIFY6b, and RcTIFY10b that responded positively to drought stress or salt stress all contained abundant abscisic acid response elements. 【Conclusion】 There are 13 RcTIFY genes in Rose, and several members of this family play active roles in drought stress and salt stress response, and abscisic acid and methyl jasmonate are potential regulatory hormones for members of this family.
作者
王昊宁
周佩
刘燕
刘梦
朱晓仪
石力匀
栗燕
逯久幸
WANG Haoning;ZHOU Pei;LIU Yan;LIU Meng;ZHU Xiaoyi;SHI Liyun;LI Yan;LU Jiuxing(College of Landscape Architecture and Art,Henan Agricultural University,Zhengzhou 450002,China;Henan Provincial Engineering Research Center for Superior Floriculture,Vegetable,and Germchit,Zhengzhou 450002,China)
出处
《河南农业大学学报》
CAS
CSCD
2022年第6期990-997,1060,共9页
Journal of Henan Agricultural University
基金
国家自然科学基金项目(32201615)
河南省教育厅重点科研项目(22A220001)。
