摘要
为明确贵州省贵阳市皂荚细菌性溃疡病的病原并筛选有效防治药剂,以发生溃疡病的皂荚果为材料,用组织分离法对病原菌进行分离并通过柯赫氏法则验证;根据生理生化特征、电镜观察结构及16S rDNA测序结果对病原菌进行鉴定;在离体条件下检测了5种药剂对病原菌生长的影响。结果表明,分离所获8株疑似致病菌,经回接后发现其中5株可引起皂角溃疡病,经鉴定,这5株均为梭叶芽孢杆菌(Bacillus toyonensis);5种供试药剂对菌株ZJ1的抑制率由高到低为:辛菌胺醋酸盐(99.38%)>石硫合剂(66.48%)>中生菌素(30.78%)>多粘类芽孢杆菌(11.91%)>春雷霉素(1.48%)。该研究首次发现Bacillus toyonensis引起皂荚溃疡病,不同药剂抑菌作用的调查结果也为防治用药的进一步筛选提供了参考。
To clarify the pathogen of bacterial canker of Gleditsia sinensis in Guiyang of Guizhou Province and select the effective bactericides, we isolated the pathogenetic bacteria from diseased G. sinensis fruit by tissue isolation method and verified Koch’s postulate. The pathogen was identified by physiological and biochemical characteristics, electron microscopic observation, and 16S rDNA sequence analysis. The effects of five bactericides on the growth of the pathogen were tested in vitro. The results showed that eight strains of the suspected pathogenic bacteria were isolated, and five of them were found to cause bacterial canker after inoculation. These five strains were identified as Bacillus toyonensis. The inhibition rates of five antibacterial agents against the strain ZJ1 from high to low were as follows: octylamine acetate(99.38%) > lime sulfur(66.48%) > zhongshengmycin(30.78%) > Bacillus polymyxa(11.91%) > chunleimycin(1.48%).In this study, Bacillus toyonensis was found to cause G. sinensis bacterial canker for the first time, octylamine acetate can be a good choice for the biological control of this disease.
作者
雷娇娇
祝静
武承旭
于存
Lei Jiaojiao;Zhu Jing;Wu Chengxu;Yu Cun(College of Forestry,Guizhou University,Guiyang Guizhou 550025,China)
出处
《中国植保导刊》
北大核心
2022年第11期14-17,21,共5页
China Plant Protection
基金
贵州省林业局基础科研项目(黔林合[2021]06)。
关键词
皂荚
细菌性溃疡病
病原鉴定
梭叶芽孢杆菌
药剂筛选
Gleditsia sinensis
bacterial canker disease
pathogen identification
Bacillus toyonensis
bactericide screening
