摘要
从健康刺参肠道分离得到一株细菌M2-4,经16S rRNA基因鉴定为鼠李糖乳杆菌,并探索了其发酵条件及对刺参的作用效果。该菌株发酵最适初始pH、温度、碳源、氮源分别为5.5~6.0、20.0~25.0℃、蔗糖、酵母提取物;最优碳源、氮源组合为蔗糖20.0 g/L、酵母提取物5.0 g/L、蛋白胨2.5 g/L;最适接种密度为4.70×10^(5)CFU/mL。在鼠尾藻粉中分别添加0、1.0×10^(5)(M5)、1.0×10^(7)(M7)、1.0×10^(9)(M9)CFU/g该菌株,进行30 d的养殖实验,各处理组刺参生长差异不显著(P>0.05),而刺参肠道中的酸性磷酸酶、总一氧化氮合酶、溶菌酶(M9除外)等酶活性显著提高(P<0.05),这表明该菌株可以显著提高刺参的非特异性免疫力。研究结果为该菌株在刺参养殖上的应用积累了基础数据。
A strain M2-4 isolated from the intestines of healthy sea cucumbers was identified as Lactobacillus rhamnosus by 16S r RNA gene and its fermentation conditions and effects on the growth and nonspecific immunity of sea cucumber were explored. The optimal initial pH, fermentation temperature, carbon source, and nitrogen source was 5.5-6.0, 20.0-25.0 ℃, sucrose and yeast extract respectively;the optimal combination of carbon and nitrogen source was sucrose 20.0 g/L, yeast extract 5.0 g/L and peptone 2.5 g/L;the optimal inoculation density was 4.70×10^(5) CFU/mL. The strain was supplemented into powder of Sargassum thunbergii at 0, 1.0×10^(5), 1.0×10^(7), 1.0×10^(9) CFU/g to feed sea cucumbers for 30 d. It was indicated that dietary M2-4 had no significant effect on the growth of sea cucumber. However, the activities of intestinal immune enzymes such as acid phosphatase, total nitric oxide synthase and lysozyme were significantly increased, which indicated that dietary M2-4 could improve the intestinal nonspecific immunity of sea cucumber. This study had accumulated basic data for the application of this strain in the farming of sea cucumbers.
作者
袁磊
宋美玲
周怡
程元秋
黎叶
邱少卿
赵彦翠
YUAN Lei;SONG Meiling;ZHOU Yi;CHENG Yuanqiu;LI Ye;QIU Shaoqing;ZHAO Yancui(School of Food Science and Technology,Shandong Business Institute,Shandong Yantai 264670,China;Jiaozhou Municipal Bureau of Agriculture and Rural Affairs,Shandong Qingdao 266300,China;Qingdao Master Biotech Co.,Ltd.,Shandong Qingdao 266319,China;School of Life Science,Ludong University,Shandong Yantai 264025,China)
出处
《饲料工业》
CAS
北大核心
2023年第2期95-101,共7页
Feed Industry
基金
山东省高等学校科技计划项目[J17KB107]
山东省自然科学基金项目[ZR2017MC045]。
关键词
刺参
乳酸菌
鉴定
发酵
应用
sea cucumber
lactic acid bacterium
identification
fermentation
application
