摘要
以薏苡仁为原料,选取酶解温度、料液比、α-淀粉酶添加量以及酶解时间4个因素进行单因素试验,利用响应面法建立数学模型,根据所得模型分析4个因素对寡糖得率的影响,最后对提取工艺进行优化。结果表明最佳工艺条件为:料液比1∶16.41(g/mL)、酶添加量0.91 g、酶解时间111.49 min、酶解温度45.46℃,其中对薏苡仁寡糖得率影响最大的因素条件为酶解温度。在该条件下,薏苡仁寡糖得率为33.01%,与模型预测值33.19%接近,该工艺条件切实可行,可用于预测薏苡仁寡糖的提取。
Firstly,the four factors of enzymatic digestion temperature,material to liquid ratio,α-amylase addition and enzymatic digestion time were selected as the raw material of coix lacryma for the single-factor test.Then using the response surface method to establish a mathematical model.The resulting model was used to analyse the effect of four factors on the oligosaccharide yield,and finally,the extraction process was optimised.According to the experiment results,the optimum process conditions were that the ratio of material and liquid was 1∶16.41(g/mL),enzyme addition was 0.91 g,enzymatic digestion time was 111.49 min,and enzymatic digestion temperature was 45.46℃.The enzymatic digestion temperature was the most influential factor on the yield of coix lacryma oligosaccharides.Under these conditions,the yield of coix lacryma oligosaccharide was 33.01%,which was close to the model prediction of 33.19%.The process conditions were practicable and could be used to predict the extraction of coix lacryma oligosaccharide.
作者
郑舒怡
左佳昕
戴鑫
高淑娟
张怡
卢旭
ZHENG Shu-yi;ZUO Jia-xin;DAI Xin;GAO Shu-juan;ZHANG Yi;LU Xu(College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002;Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch,Fuzhou 350002,China;China-Ireland International Cooperation Centre for Food Material Science and Structure Design,,Fuzhou 350002,China;Key Laboratory of Subtropical Characteristic Fruits,Vegetables and Edible Fungi Processing(Co-construction by Ministry and Province),Ministry of Agriculture and Rural Affairs,Fuzhou 350002,China)
出处
《化学试剂》
CAS
北大核心
2023年第2期90-97,共8页
Chemical Reagents
基金
国家自然科学基金面上基金项目(32172151)
福建省对外合作项目(2021I0007)
“中爱国际合作食品物质学与结构设计研究中心”项目(KXGH17001)
福建农林大学杰出青年科研人才计划项目(xjq201811)
福建农林大学科技创新专项基金项目(CXZX2018069,CXZX2019095G,CXZX2020120A)。
关键词
薏苡仁
寡糖
酶解
响应面
提取过程
Coix lacryma
oligosaccharides
enzymatic digestion
response surface
extraction process
