摘要
目的探究《中华人民共和国药典》(2020年版)(简称“《中国药典》”)收载的清蒸炮制法,在不同炮制时间(0~48 h)下对何首乌中主要化学成分含量的影响。方法制备《中国药典》清蒸样品;建立超高效液相色谱-三重四极杆质谱(UPLC-MS/MS)测定何首乌中26种成分的分析方法并进行检测。结果建立了准确可靠的UPLC-MS/MS测定何首乌中蒽醌、二苯乙烯苷、黄酮、酚类化合物含量的方法;随着蒸制时间的延长,26种成分的含量发生了明显变化:游离蒽醌类成分(大黄素、大黄素甲醚)先降低后升高,结合蒽醌类成分(大黄素-8-O-β-D-葡萄糖苷、大黄素甲醚-8-O-β-D-葡萄糖苷、大黄素-1-O-葡萄糖苷、大黄素-6-O-葡萄糖苷)、大黄酸、大黄素-1-甲醚、cis-THSG、虎杖苷、表儿茶素和芦丁先升高后降低,大黄素二蒽酮、大黄素-8-O-(6’-甲基丙二酰)吡喃葡萄糖苷、大黄素甲醚-8-O-(6’-甲基丙二酰)吡喃葡萄糖苷、trans-THSG、白藜芦醇、2,3,5,4’-四羟基二苯乙烯-2-O-(2”-O-阿魏酰)-β-D-吡喃葡萄糖苷、儿茶素和决明酮-8-O-β-D-葡萄糖苷降低,酚类成分升高。结论本方法操作简单、准确度高、重复性好,可以用于何首乌中26种化学成分的定量检测分析。制首乌中26种成分的含量与蒸制时间密切相关,蒽醌类、二苯乙烯苷类、黄酮类、酚类发生规律性变化,对何首乌质量产生重要影响。
Objective To explore the effects of duration of steaming(0~48 h)and processing methods recorded in“Chinese Pharmacopoeia”on the contents of main chemical components of Polygoni Multiflori Radix(PM).Methods Pharmacopoeia-defined steamed samples were prepared before an ultra-high performance liquid chromatographytriple quadrupole mass spectrometry(UPLC-MS/MS)analytical method was established for determination of the 26components in PM.Results An accurate and reliable UPLC-MS/MS method was established for the determination of anthraquinone,stilbene glycosides,flavonoids and phenolic compounds in PM.With the extension of steaming time,the contents of 26 components changed significantly.The contents of free anthraquinone components(emodin,physcion)decreased before they increased,while those of combined anthraquinone components(emodin-8-O-β-D-glucoside,Physcion--8-O-β-D-glucoside,emodin-1-O-glucoside,emodin-6-O-glucoside),rhein,hydroxyemodin,emodin-1-methyl,cis-THSG,polydatin,epicatechin and rutin increased first and then decreased.The contents of emodin dianthrone,emodin-8-O-(6’-methylmalonyl)-glucopyranoside,physcion-8-O-(6’-methylmalonyl)-glucopyranoside,trans-THSG,resveratrol,2,3,5,4’-tetrahydroxystilbene-2-0-(2”-O-feruloyl)-β-D-glucopyranoside,catechin and torachrysone-8-O-β-D-glucopyranoside decreased,while those of phenolic components increased.Conclusion This method is simple,accurate and repeatable,which can be used for quantitative detection and analysis of 26 chemical components in PM.The contents of the 26 components in PM are closely related to the duration of steaming.Anthraquinones,stilbene glycosides,flavonoids and phenols undergo regular changes,which have an impact on the quality of PM.
作者
李妍怡
王莹
张南平
杨建波
刘越
汪祺
张玉杰
魏锋
马双成
LI Yanyi;WANG Ying;ZHANG Nanping;YANG Jianbo;LIU Yue;WANG Qi;ZHANG Yujie;WEI Feng;MA Shuangcheng(School of Chinese Materia medica,Beijing University of Chinese Medicine,Beijing 102400,China;National Institutes for Food and Drug Control,Beijing 100050,China)
出处
《中国药物警戒》
2022年第12期1295-1302,共8页
Chinese Journal of Pharmacovigilance
基金
国家自然科学基金资助项目(81973476)。
