摘要
目的探讨长链非编码RNA 01287(LncRNA 01287)通过靶向调节微小RNA(miR-298)对胃癌细胞生物活性的影响,以及对信号传导和转录激活因子3(STAT3)通路的调节作用。方法胃癌SGC-7901细胞随机分为对照组、模拟物阴性对照组(mimic-NC组)、模拟物组(mimic组)、抑制剂阴性对照组(inhibitor-NC组)以及抑制剂组(inhibitor组),除对照组外,其余组分别转染mimic NC、mimic、inhibitor NC以及inhibitor,qRT-PCR检测LncRNA 01287和miR-298的表达水平,MTT法检测细胞存活率、划痕实验检测细胞迁移能力、侵袭实验检测细胞侵袭能力,双荧光素酶报告基因法检测LncRNA 01287和miR-298以及miR-298和STAT3的靶向关系,蛋白印迹法检测p-STAT3、上皮钙粘蛋白(E-cad)、神经钙粘蛋白(N-cad)和波形蛋白(Vimentin)相对表达量。结果LncRNA 01287靶向作用于miR-298,miR-298靶向作用于STAT3;与mimic-NC组比较,mimic组LncRNA 01287表达水平、划痕愈合率、侵袭细胞数以及p-STAT3、N-cad和Vimentin蛋白相对表达量升高,miR-298表达水平以及E-cad蛋白相对表达量降低(P<0.05);与inhibitor-NC组比较,inhibitor组LncRNA 01287表达水平、细胞存活率、划痕愈合率、侵袭细胞数以及p-STAT3、N-cad和Vimentin蛋白相对表达量降低,miR-298表达水平以及E-cad蛋白相对表达量升高(P<0.05)。结论LncRNA 01287通过靶向调节miR-298表达激活STAT3信号通路,促进胃癌细胞生物活性。
Objective To investigate the effects of long non-coding RNA 01287(LncRNA 01287)on the biological activity of gastric cancer cells and the regulation of signal transducer andactivator of transcription3(STAT3)pathway through targeted regulation of miR-298.Methods Gastric cancer SGC-7901 cells were randomly divided into the control group,mimic-NC group,mimic group,inhibitor-NC group and inhibitor group.Except the control group,the other groups were transfected with mimic NC,mimic,inhibitor NC and inhibitor respectively.The expression levels of LncRNA 01287 and miR-298 were detected by qRT-PCR.MTT assay was used to detect cell survival rate,Nick assay was used to detect cell migration ability,Transwell assay was used to detect cell invasion ability,and dual luciferase reporter gene assay was used to detect the targeting relationship between LncRNA 01287 and miR-298 as well as miR-298 and STAT3.Western blot was used to detect the relative protein expression levels of p-STAT3,epithelial cadherin(E-cad),neurocadherin(N-cad)and Vimentin.Results LncRNA 01287 targets miR-298 and miR-298 targets STAT3.Compared with mimic-NC group,LncRNA 01287 expression level,scratch healing rate,number of invaded cells and relative protein expression levels of p-STAT3,N-cad and Vimentin were increased in mimic group,while miR-298 expression level and relative protein expression level of E-cad were decreased(P<0.05).Compared with inhibitor NC group,the expression level of LncRNA 01287,cell survival rate,scratch healing rate,number of invaded cells and relative protein expression levels of p-STAT3,N-cad and Vimentin in the inhibitor group were decreased,the expression level of miR-298 and the relative expression of E-cad protein were increased(P<0.05).Conclusion LncRNA 01287 stimulates the STAT3 signaling pathway by targeting miR-298 expression and promotes the biological activity of gastric cancer cells.
作者
郑豪
李东亮
朱清海
ZHENG Hao;LI Dongliang;ZHU Qinghai(Zhumadian Central Hospital,Zhumadian,463000)
出处
《实用癌症杂志》
2023年第2期202-206,216,共6页
The Practical Journal of Cancer
