摘要
目的:以鼠伤寒沙门氏菌CGMCC 1.1190为对象,研究其经柠檬酸反复胁迫处理诱导的抗酸性机理。方法:CGMCC 1.1190在经柠檬酸调节到pH为2.5的TSB培养基中胁迫处理并转接12次培养后,获得的抗酸性菌株。采用iTRAQ技术对CGMCC 1.1190抗酸性菌株的蛋白组进行分析,通过GO功能注释和富集对差异蛋白进行聚类分析,以及KEGG注释和富集对差异蛋白参与的通路进行联合分析。结果:iTRAQ技术结果显示CGMCC 1.1190抗酸性菌株共2373个蛋白,其中195个差异蛋白中,95个显著下调蛋白和100个显著上调蛋白。GO分析和KEGG分析结果显示CGMCC 1.1190抗酸性菌株高表达应激蛋白相关基因;高表达细胞膜相关蛋白可促进CGMCC 1.1190菌体细胞膜的完整性;高表达鞭毛相关蛋白可增强CGMCC 1.1190菌体的运动能力并促进保护性生物被膜的形成;高表达双组分系统相关蛋白来响应酸信号,通过调控酸激蛋白的产生增强CGMCC 1.1190菌体的抗酸性;低表达ABC转运系统相关蛋白来降低细胞膜对H+的通透性,达到保护菌体的作用;高表达能量代谢途径相关蛋白为CGMCC 1.1190菌体应对酸胁迫提供能量。结论:CGMCC 1.1190在经pH为2.5的柠檬酸胁迫后,能通过蛋白水平的表达变化启动一系列应答机制并生存下来,通过合成一些蛋白质来增强CGMCC 1.1190的抗酸性。
Objective:To investigate the mechanism of acid resistance induced by repeated stress treatment with citric acid for Salmonella typhimurium CGMCC 1.1190.Methods:The acid-resistant strain of CGMCC 1.1190 was obtained after stress treatment in TSB liquid medium adjusted to pH2.5 by citric acid and transferred and incubated 12 times.The proteome of the acid-resistant strain of CGMCC 1.1190 was analyzed based on iTRAQ technology,with clustering analysis of the differential proteins by GO functional annotation and enrichment for biological processes,molecular functions,and cellular components,and KEGG annotation and enrichment for combined analysis of the pathways involved in the differential proteins.Results:The iTRAQ results showed that the CGMCC 1.1190 acid-resistant strain had 2373 proteins,of which 195 differential proteins,including 95 significantly down-regulated proteins and 100 significantly up-regulated proteins.GO analysis and KEGG analysis showed that CGMCC 1.1190 acid-resistant strains highly express stress proteinrelated genes,which could contribute CGMCC 1.1190 to enhance acid resistance by synthesizing some proteins in response to external stimulation.The high express cell membrane-related proteins could increase the integrity of CGMCC 1.1190 cell membrane,and the high expression of flagellar-related proteins could enhance the motility and increase the formation of protective biofilm of CGMCC 1.1190.The high expression of two-component system-related proteins could enhance acid resistance of CGMCC 1.1190 by regulating the production of acid kinins.The low expression of ABC transport system-related proteins could reduce the permeability of the cell membrane to H+to protect the bacterium,and the proteins related to the energy metabolism pathway were highly expressed to provide energy and maintain the homeostasis of the CGMCC 1.1190 bacterium in response to acid stress.Conclusion:CGMCC 1.1190 could initiate a series of response mechanisms and survive after citric acid stress at pH2.5 due to some changes in protein expression.
作者
张丽君
李琳琼
尹大成
高瑀珑
ZHANG Lijun;LI Linqiong;YIN Dacheng;GAO Yulong(College of Food Science and Engineering,Nanjing University of Finance and Economics,Collaborative Innovation Center for Modern Grain Circulation and Safety,Nanjing 210023,China)
出处
《食品工业科技》
CAS
北大核心
2023年第4期1-11,共11页
Science and Technology of Food Industry
基金
粮食流通与安全国际合作联合实验室建设项目
国家自然科学基金面上项目(31371864)。
