摘要
为明确新疆库尔勒地区梨火疫病致病菌,并筛选有效防治药剂。采用平板划线法获得分离物,结合致病性测定、形态学特征与分子生物学技术综合鉴定致病菌,采用“药液-菌液共培养法”与“抑菌圈法”测定16种杀菌剂对其室内毒力。鉴定分离物为Erwinia amylovora。16种杀菌剂对梨火疫病菌均有抑制作用。其中,0.3%四霉素AS抑菌效果最好,EC 50为2.968μg/mL。研究结果对梨火疫病田间药剂防治提供科学依据。
To identify the pathogens of pear fire blight in Korla of Xinjiang Uygur Autonomous Region and screen out the effective bactericides,the pure isolates were obtained by plate scribing method,and the pathogenic bacteria was identified by the combined methods of pathogenicity determination experiments,morphological characteristics and molecular biological techniques.The toxicity tests of the 16 kinds of bactericides were determined by co-cultivation method of bactericide liquid and bacterial liquid and inhibition zone method,and the EC 50 value were analyzed.The results showed that the suspected pathogen was identified as Erwinia amylovora.All the 16 bactericides had obvious inhibitory effects on pear fire blight.Among them,tetramycin 0.3%AS had the best bacteriostatic effect,with EC 50 of 2.968μg/mL.This study provides a scientific basis for the field chemical control of pear fire blight.
作者
陈晓晓
艾尼赛·赛米
粟神强
贾玉凤
刘琦
陈晶
CHEN Xiaoxiao;Anisa·Saimi;SU Shenqiang;JIA Yufeng;LIU Qi;CHEN Jing(College of Agriculture,Xinjiang Agricultural University,Key Laboratory of the Pest Monitoring and Safety Control of Crops and Forests,Urumqi 830052,China)
出处
《西北农业学报》
CAS
CSCD
北大核心
2023年第3期468-478,共11页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家自然科学基金(31860477)
3%、12%噻霉铜水分散粒剂防治梨树苹果枝枯病药效试验(2520HXKT7)。
关键词
梨火疫病
病原鉴定
杀菌剂
毒力测定
药剂筛选
Pear fire blight
Pathogen identification
Bactericide
Toxicity test
Bactericide screening
