摘要
目的分析新疆哈密地区500例急性呼吸道感染患儿13种病毒多重检测的调查结果。方法选取2018年1月-2021年1月入院就诊的急性呼吸道感染患儿500例,采用多重逆转录聚合酶链反应(RT-PCR)技术扩增,通过毛细管电泳,检测13种呼吸道感染病毒:人呼吸道合胞病毒(RSV)、人鼻病毒(hRV)、呼吸道腺病毒(AdV)、甲、乙型流行性感冒病毒(Inf A、Inf B)、副流感病毒(PIV 1/2/3)、人肠道病毒(hEV)、人偏肺病毒(hMPV)、人冠状病毒(hCoV 229E/OC43)、人博卡病毒(hBoV),并与直接测序法进行比对。结果500份样本中阳性样本379份(75.80%),检出率前三位分别为RSV(19.40%)、hRV(16.00%)、Inf B(12.60%);<1岁组、1~3岁组、>3岁组,呼吸道病毒检测阳性率分别为84.97%、77.47%、65.45%,三组比较差异有统计学意义(P<0.05),RSV检出率在<1岁组最高,Inf A和Inf B检出率在>3岁组最高;春季组、夏季组、秋季组、冬季组呼吸道病毒检测阳性率分别为74.05%、63.73%、77.24%、84.03%,四组比较差异有统计学意义(P<0.05),RSV、PIV 3、hMPV检出率在冬季组最高,AdV检出率在春季组最高。结论RSV是新疆哈密地区急性呼吸道感染患儿的主要感染病毒,呼吸道病毒分布与患儿年龄、发病季节有关。
OBJECTIVE To investigate and analyze multiple detection of 13 kinds of viruses in 500 children with acute respiratory tract infection in Hami of Xinjiang.METHODS A total of 500 children with acute respiratory tract infection treated in the hospital between Jan 2018 and Jan 2021 were enrolled.Thirteen kinds of respiratory infection viruses including human respiratory syncytial virus(RSV),human rhinovirus(hRV),respiratory adenovirus(AdV),influenza A and B viruses(Inf A,Inf B),parainfluenza virus(PIV 1/2/3),human enterovirus(hEV),human metapneumovirus(hMPV),human coronavirus(hCoV 229E/OC43)and human Boca virus(hBoV)were detected by multiple reverse transcription polymerase chain reaction(RT-PCR)amplification and capillary electrophoresis.And the results were compared with those by direct sequencing method.RESULTS Of the 500 samples,379 samples were positive(75.80%),and the top three detection rates were RSV(19.40%),hRV(16.00%)and Inf B(12.60%).The differences in positive rates of the respiratory virus among<1 year group,1-3 years group and>3 years group were significant(84.97%,77.47%,65.45%)(P<0.05).The detection rate of RSV was the highest in<1 year group,and the detection rates of Inf A and Inf B were the highest in>3 years group.The differences in positive rates of respiratory viruses among the spring group,summer group,autumn group and winter group were significant(74.05%,63.73%,77.24%,84.03%)(P<0.05).The detection rates of RSV,PIV 3,and hMPV were the highest in the winter group,and detection rate of AdV was the highest in spring group.CONCLUSION RSV is the main infection virus in children with acute respiratory infection in Hami of Xinjiang.The distribution of respiratory viruses is related to age and onset season in children.
作者
马宏伟
史传见
李平
于丽
杨荣
MA Hong-wei;SHI Chuan-jian;LI Ping;YU Li;YANG Rong(Hami Central Hospital,Hami,Xinjiang 839000,China)
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2022年第23期3643-3647,共5页
Chinese Journal of Nosocomiology
基金
哈密地区科学研究与技术开发基金资助项目(ZD20150013)。
关键词
急性呼吸道感染
病毒
多重核酸检测
毛细管电泳
流行病学调查
哈密
儿童
Acute respiratory tract infection
Virus
Multiple nucleic acid detection
Capillary electrophoresis
Epidemiological survey
Hami
Child
