摘要
目的探讨瓣状核酸内切酶-1(FEN1)、转录因子Ⅱⅰ假基因23(GTF2IP23)、赖氨酸特异性去甲基化酶4A(KDM4A)在乳腺癌组织中的表达及相关性。方法选取2020年7月至2021年8月进行手术的女性乳腺癌患者72例及同期进行手术的乳腺良性肿瘤患者70例,收集乳腺癌患者手术切除癌组织、距癌组织5 cm癌旁组织标本及乳腺良性肿瘤患者肿瘤病理组织,连续切片后做免疫组化标记、进行免疫组化染色。反转录-实时荧光定量聚合酶链反应(RT-qPCR)法检测FEN1表达,Western blot法检测GTF2IP23、KDM4A表达,分析FEN1、GTF2IP23、KDM4A在乳腺癌组织中的表达相关性及联合检测对乳腺癌患者预后的预测价值。结果与癌旁组织相比,良性肿瘤组织、乳腺癌组织中FEN1、GTF2IP23、KDM4A表达较高(P<0.05);乳腺癌组织中FEN1、GTF2IP23、KDM4A表达高于癌旁组织(P<0.05)。乳腺癌组织中FEN1、GTF2IP23、KDM4A表达与年龄、肿瘤直径、病理分期、淋巴结转移、脉管侵犯、分化情况密切相关(P<0.05)。乳腺癌组织中FEN1与GTF2IP23、KDM4A表达呈正相关(r=0.404、0.553,均P=0.001);GTF2IP23与KDM4A表达也呈正相关(r=0.582,P=0.001)。与FEN1、GTF2IP23、KDM4A单项检测相比,三项联合检测对乳腺癌患者预后预测的灵敏度提高,特异度降低;FEN1、GTF2IP23、KDM4A的曲线下面积(AUC)分别为0.691、0.659、0.708(P<0.05)。结论FEN1、GTF2IP23、KDM4A在乳腺癌组织中呈高表达,三者具有相关性,同时与乳腺癌患者病理特征密切相关,能够较好预测乳腺癌患者的预后,为临床诊断及治疗乳腺癌提供理论依据。
Objective To explore the expression and relevance of flap endonuclease-1(Fen1),transcription factor Ⅱⅰpseudogene 23(gtf2ip23),lysine specific demethylase 4a(kdm4a)in breast cancer.Methods Selected female surgery in July 2020 to August 2021 and benign breast cancer patients,collected breast cancer patients surgical resection of breast cancer tissue,adjacent tissue specimens 5 cm from cancer tissue and breast benign tumor tumor pathological tissue,continuous sections after immunohistochemical marks,immunohistochemical staining.FEN1 expression was detected by RT-qPCR,GTF2IP23 and KDM4A expression was detected by Western blot,and the correlation of FEN1,GTF2IP23 and KDM4A expression in breast cancer tissues and the predictive value of combined testing in breast cancer patients were analyzed.Results FEN1,GTF2IP23,and KDM4A expressions were higher in benign and breast cancer tissues(P<0.05)and FEN1,GTF2IP23,and KDM4A in breast cancer than benign breast cancer tissue(P<0.05).The expression of FEN1,GTF2IP23 and KDM4A in breast cancer was closely correlated with age,tumor diameter,pathological stage,lymph node metastasis,vascular invasion and differentiation(P<0.05).A positive expression of FEN1 and GTF2IP23 in breast cancer tissues(r=0.404,P=0.001),FEN1 and KDM4A expression(r=0.553,P=0.001)and GTF2IP23 and KDM4A expression(r=0.582,P=0.001).Compared with the single tests of FEN1,GTF2IP23,and KDM4A,the three combined tests had high sensitivity and low specificity in the prognosis prediction of breast cancer patients,with AUC values of 0.691,0.659,and 0.708 for FEN1,GTF2IP23,and KDM4A,respectively(P<0.05).Conclusion FEN1,GTF2IP23 and KDM4A are highly expressed in breast cancer tissues.They are correlated and closely related to the pathological characteristics of breast cancer patients.They can better predict the prognosis of breast cancer patients and provide a theoretical basis for clinical diagnosis and treatment of breast cancer.
作者
谭秋芬
胡惠军
TAN Qiufen;HU Huijun(The Third People's Hospital of Huizhou,Huizhou,Guangdong 516002,China)
出处
《国际检验医学杂志》
CAS
2023年第3期311-315,321,共6页
International Journal of Laboratory Medicine
基金
惠州市科技计划项目(20200806)。
