摘要
为探讨旱、盐胁迫对不同海拔、生长年限和贮藏时间的药用大黄种子萌发和幼苗生长的影响,以采自陕西汉中镇巴县的药用大黄种子为研究对象,纯净水(CK)为对照组,置于用以模拟干旱和盐胁迫的不同渗透势水平(-0.3和-0.5 MPa)的PEG和NaCl处理下,每日记录种子萌发个数并观察幼苗生长情况。结果表明:相比低和高海拔的种子,中海拔1650 m处采集的种子萌发率更高,且海拔1650和1300 m处的种子对干旱和盐胁迫的耐受力强;随着胁迫浓度的增加,干旱胁迫对药用大黄幼苗生长的抑制作用极其显著,在-0.5 MPa PEG处理下所有幼苗均死亡;不同海拔、不同生长年限及不同贮藏期的种子内源赤霉素含量无明显差异,而赤霉素浸种后种子活力和内源赤霉素浓度显著升高;室温贮藏1年的大黄种子发芽率、活力、耐盐性显著低于新采集的种子,而3年生植株种子发芽率、活力、耐盐性均高于2年生且耐旱性更强。综上可知,药用大黄种子不适宜长期室温贮藏,中海拔以及轻度盐胁迫有助于药用大黄种子萌发及幼苗生长,且3年生药用大黄种子质量高于2年生。
We explored the effects of drought and salt stresses on seed germination and seedling growth of Rheum officinale Baill. at different altitudes, growth years and storage periods, with seeds collected from Zhenba County, Shaanxi Province. PEG and NaCl treatments with two osmotic potential levels(-0.3 and-0.5 MPa) were used to simulate drought stress and salt stress, respectively. The number of seed germination was recorded daily and the growth of seedlings was observed. The results showed that the germination rate of seeds collected at the middle altitude of 1650 m was higher than that of seeds collected at the low and high altitudes. Seeds collected at the altitudes of 1650 and 1300 m were more tolerant to drought and salt stresses. With the increases of stress concentration, the inhibitory effect of drought stress on seedling growth was extremely significant. All seedlings died under-0.5 MPa PEG treatment. There was no significant difference in the endogenous GA3content of seeds collected at different altitudes, growth years and storage periods. Seed vigor and endogenous GA3concentration increased significantly after gibberellin soaking. The germination rate, vigor, and salt tolerance of seeds stored for one year at room temperature were significantly lower than that of newly collected seeds, while the germination rate, vigor, and salt tolerance of seeds from three-year-old plants were higher than those from two-year-old plants. Drought tolerance of seeds from three-year-old plants was stronger. In summary, R. officinale seeds are not suitable for long-term storage at room temperature. Medium altitude and mild salt stress are beneficial to seed germination and seedling growth of R. officinale. Seed quality of three-year-old plants is higher than that of two-year-old plants.
作者
谢丰璞
王楠
高静
张岗
葛甜甜
张亚丽
成世强
宋忠兴
唐志书
XIE Feng-pu;WANG Nan;GAO Jing;ZHANG Gang;GE Tian-tian;ZHANG Ya-li;CHENG Shi-qiang;SONG Zhong-xing;TANGZhi-shu(State Key Laboratory of Research&Development of Characteristic Qin Medicine Resources(Cultivation),Co-construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi&Education Ministry,Shaanxi University of Chinese Medicine,Xianyang 712083,Shaanxi,China;Key Laboratory for Research of"Qin Medicine"of Shaanxi Administration of Traditional Chinese Medicine,College of Pharmacy,Shaanxi University of Chinese Medicine,Xi’an 712046,China;Zhenba County Science and Technology Progress Promotion Center,Hanzhong 723000,Shaanxi,China;China Academy of Chinese Medical Sciences,Beijing 100700,China)
出处
《生态学杂志》
CAS
CSCD
北大核心
2023年第1期18-28,共11页
Chinese Journal of Ecology
基金
国家自然科学基金(81973430)
陕西省教育厅协同创新中心项目(20JY013)
陕西省重点产业创新链项目(2021ZDLSF04-01)
现代农业产业技术体系建设专项(CARS-21)资助。
关键词
渗透胁迫
海拔
生长年限
贮藏期
种子萌发
幼苗生长
赤霉素
osmotic stress
altitude
growth year
storage period
seed germination
seedling growth
gibberellin
