摘要
以云南8个地理种源野生滇重楼为材料,采用高效液相色谱法测定皂苷I、II、VI、VII含量,综合根茎生物量及年增长率进行分析。结果表明:不同种源滇重楼根茎皂苷含量呈极显著差异,变异幅度为2.145~15.749 mg/g,皂苷含量为普洱景东>玉溪澄江>文山丘北>楚雄大姚>曲靖马龙、昭通镇雄>大理剑川>丽江永胜;各种源均以薯蓣皂苷为主,偏诺皂苷含量为辅;总皂苷含量与经度呈显著正相关性、与纬度呈显著负相关性,皂苷I与经度和年均温呈显著正相关关系,总皂苷与海拔相关系数为-0.371、与年均温和年降水量相关系数分别为0.668和0.672;总皂苷与皂苷Ⅰ含量呈高度正相关;大理剑川种源滇重楼根茎生长速率最高,排序为大理剑川>丽江永胜>普洱景东>玉溪澄江>楚雄大姚>文山丘北、曲靖马龙>昭通镇雄。聚类分析表明,8个种源可以划分为3个类群,中皂苷、低生长速率类群,高皂苷、高生长速率类群,低皂苷、高生长速率类群。
The contents of saponins I,II,VI and VII of 8 wild Paris polyphylla in Yunnan province were determined by HPLC,and the rhizome biomass and annual growth rate were analyzed.The findings indicated that there were significant differences in the content of saponins among these provenances.The variation range of total saponins content was 2.145–15.749 mg/g.The saponins content of 8 provenances from high to low were Pu’er Jingdong,Yuxi Chengjiang,Wenshan Qiubei,Chuxiong Dayao,Qujing Malong,Zhaotong Zhenxiong,Dali Jianchuan and Lijiang Yongsheng.Diosgenin and pinoside were the main components of saponins in these provenances.The content of total saponin was highly positive correlated with longitude and a significant negative correlation with latitude.Saponin I was significant positive correlation with longitude and annual average temperature.The correlation coefficients between total saponins and altitude,annual average temperature and annual precipitation were-0.371,0.668 and 0.672 respectively.There was a high positive correlation between total saponins and saponin I.The rhizome growth rate of these provenances from high to low were were Dali Jianchuan,Lijiang Yongsheng,Pu’er Jingdong,Yuxi Chengjiang,Chuxiong Dayao,Wenshan Qiubei,Qujing Malong and Zhaotong Zhenxiong.Cluster analysis showed that the 8 provenances could be divided into 3 groups:medium saponin and low growth rate group,high saponin and high production rate group,low saponin and high growth rate group.The provenances from Pu’er Jingdong and Yuxi Chengjiang were superior provenances with high saponins.
作者
徐永艳
李昆
焦蓉
汪琼
王占娣
石冰杰
徐荣
Xu Yongyan;Li Kun;Jiao Rong;Wang Qiong;Wang Zhandi;Shi Bingjie;Xu Rong(Southwest Forestry University,Kunming Yunnan 650233,China;Research Institute Resources Insects,China Academy of Forestry,Kunming Yunnan 650224,China;Yuxi Normal University,Yuxi Yunnan 653100,China)
出处
《西南林业大学学报（自然科学）》
CAS
北大核心
2023年第1期36-41,共6页
Journal of Southwest Forestry University：Natural Sciences
基金
国家林业公益性行业科研专项(201304202)资助
云南省基础研究项目(2020-530622-01-01-034399)资助
云南省地方本科高校基础研究联合专项(202101BA070001-055)资助
云南省高校玉溪特色农业资源发掘与利用重点实验室资助。
关键词
滇重楼
林下资源
皂苷
种源
评价
筛选
Paris polyphylla var.yunnanensis
understory resources
steroidal saponin
provenance
evaluation
screen
