摘要
目的:获得超高压提取红枣中核苷类化合物的最佳工艺。方法:采用响应面法(Response surface methodology,RSM),优化提取温度、压力、时间和液料比4个因素对提取率的影响,采用高效液相色谱法(High-performance liquid chromatography,HPLC)对提取物中核苷类化合物进行定性定量分析。结果:最佳提取条件为提取温度46.50℃、压力342.00 MPa、液料比50 mL/g、时间17.00 min,最佳条件下的提取率为631.73μg/g DW。采用HPLC法共检测出11种核苷类化合物,分别为尿嘧啶、胞苷、尿苷、环磷酸鸟苷、黄嘌呤、次黄嘌呤、鸟嘌呤、鸟苷、环磷酸腺苷、腺苷和腺嘌呤。核苷提取物对DPPH和ABTS自由基具有良好的清除作用,其IC_(50)值分别为0.22 mg/mL和0.25 mg/mL。研究结果将为新疆红枣的品质评价及功能性食品的开发提供基础数据。
Objective:To obtain the best process for extracting nucleoside compounds in jujube.Methods:Response surface methodology was used to optimize the effects of extraction temperature,pressure,time and liquid to material ratio on the extraction rate,and nucleosides in the extracts were analyzed qualitatively and quantified by HPLC(High-performance liquid chromatography,HPLC).Result:The optimal extraction conditions were temperature of 46.50℃,pressure of 342.00 MPa,and the liquid material ratio of 50 mL/g for 17.00 min.The extraction rate under the optimal conditions was 631.73g/g DW.A total of 11 compounds were detected by HPLC:uracil,cytidine,uridine,cGMP,xanthine,hypoxanthine,guanine,guanosine,cAMP,adenosine and adenine.The nucleoside extracts showed good antioxidant activity against DPPH and ABTS radicals with IC_(50)values of 0.22 mg/mL and 0.25 mg/mL,respectively.The results would provided helpful knowledge for multipurpose use and quality evaluation of jujube.
作者
叶林
李璐
宋丽军
白冰瑶
YE Lin;LI Lu;SONG Lijun;BAI Bingyao(College of Food Science and Engineering,Tarim University,Construction Corps Key Laboratory of Special Agricultural Products Further Processing in Southern Xinjiang,Alar 843300,China;College of Food and Biological Engineering,Bengbu University,Bengbu 233000,China)
出处
《食品科技》
CAS
北大核心
2022年第11期191-198,共8页
Food Science and Technology
基金
新疆生产建设兵团南疆重点产业创新发展支撑计划项目(2018DB002)
塔里木大学校长基金项目(TDZKSS201908)。
关键词
红枣
核苷
响应面
超高压提取
抗氧化活性
jujube
nucleosides
response surface
ultra-high pressure extraction
antioxidant activity
