摘要
芜菁(Brassica campestris L.ssp.rapifera Matag syn.B.rapa L.)是十字花科芸薹属园艺作物,其下胚轴和主根膨大形成肉质直根。PIN(Pin-formed)是一种植物特异性蛋白,作为生长素的输出载体,介导生长素的极性运输形成生长素不对称分布,从而在植物生长发育过程中起重要作用,然而目前在芜菁中还缺乏对PIN蛋白的系统研究。本研究基于芜菁及其他植物基因组,结合芜菁和普通白菜下胚轴不同发育时期转录组数据,通过生物信息学方法,对芜菁PIN基因家族进行了鉴定,并进行了蛋白结构及基因结构分析、系统发育分析、共线性分析和表达分析。结果表明,芜菁中共有13个BcPIN,数量多于拟南芥和甜菜,但略少于萝卜、胡萝卜和水稻;不同物种间同种类PIN基因数量存在差异;芜菁PIN家族与拟南芥、萝卜PIN家族基因进化关系较近,与水稻PIN家族基因关系较远,分布在4条染色体上,包括7个共线性基因对;BcPINs启动子含有多种顺式作用元件,受多种信号调控,但不同BcPINs的顺式元件种类存在差异;BcPINs的氨基酸个数在270~661之间,分子量在29.81~70.92 kD之间,大多为碱性疏水蛋白,均含有7~10个跨膜结构域,包含4~10个保守基,定位在膜上;7个BcPIN在芜菁下胚轴不同发育时期的表达趋势与普通白菜存在差异,可能参与芜菁肉质直根的形成过程。据此,本研究鉴定了芜菁中的PIN基因家族,并对其功能进行了预测,为芜菁的分子设计育种和进一步研究植物贮藏器官发育机制提供了依据。
Turnip(Brassica campestris L.ssp.rapifera Matag syn.B.rapa L.)is a variety of horticultural crops in the Brassica genus of Brassicaceae family whose hypocotyls and main roots enlarge to form fleshy taproots.PIN(pin-formed)is a kind of plant-specific protein.As an auxin efflux carrier,PINs mediate auxin polar transport to form an asymmetric distribution,playing an important role in plant growth and development.However,there is a lack of systematic studies on PIN proteins in turnip at present.Based on the genomes of turnip and other plant species and combined the transcriptomic data of turnip and pakchoi hypocotyls at different development stages,this study identified the members of PIN gene family in turnip,and performed protein and gene structure analysis,phylogenetic analysis,synteny analysis,as well as expression analysis by using bioinformatics methods.The results showed that there were 13 BcPINs in turnip,more than those in Arabidopsis thaliana and Raphanus sativus,but less than those in Raphanus sativus,Daucus carota and Oryza sativa;the numbers of a same kind of PINs were different among different species;BcPINs had a close evolutionary relationship with PINs in Arabidopsis thaliana and Raphanus sativus,but a far evolutionary relationship with PINs in Oryza sativa,and were distributed on 4 chromosomes,including 7 collinear gene pairs;the promoter sequence of BcPINs have multiple cis-acting elements,and were regulated by multiple external signals,but different BcPINs have different cis-acting elements;BcPINs contained 270~661 amino acid residues with molecular weights ranging from 29.81 to 70.92 kD,and most of BcPINs were basic and hydrophobic proteins containing 7 to 10 transmembrane domains as well as 4 to 10 conserved motifs,and were located on the membrane;the expression profiles of 7 BcPINs in turnip hypocotyls at different development stages were different from those in pakchoi.According to these results,this study identified the PIN gene family members in turnip,and predicted their possible functions,laying a foundation for molecular design breeding in turnip as well as the further research on the development mechanisms of plant storage organs.
作者
刘锐诚
梁雨薇
孙福辉
黄鹂
Liu Ruicheng;Liang Yuwei;Sun Fuhui;Huang Li(College of Agriculture and Biotechnology,Zhejiang University,Hangzhou,310058)
出处
《分子植物育种》
CAS
北大核心
2023年第2期349-359,共11页
Molecular Plant Breeding
基金
浙江省农业新品种选育重大科技专项(2021C02065)
浙江省重点研发领雁计划项目(2022C02030,2022C02032)共同资助。
